Accumulation and Excretion Dynamics and Purification of Lipase from Bacteria Pseudomonas aeruginosa A12 Culture Medium

Accumulation and Excretion Dynamics and Purification of Lipase from Bacteria Pseudomonas aeruginosa A12 Culture Medium

A highly active and electrophoretically homogeneous enzyme with MM 31 kDa and specific lipase activity 1009 U/mg was isolated and purified from bacteria Pseudomonas aeruginosa A12 culture medium using ion-exchange and hydrophobic chromatography. The physicochemical properties of the isolated lipase...

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Bibliographic Details
Published in:Chemistry of natural compounds Vol. 55; no. 6; pp. 1123 - 1126
Main Authors: Berdiev, N. Sh, Ziyavitdinov, Zh. F., Bozorov, S. S., Ishimov, U. Zh, Olimzhonov, Sh. S., Fazliddinov, Sh. Zh, Salikhov, Sh. I.
Format: Journal Article
Language:English
Published: New York Springer US 01-11-2019
Springer
Springer Nature B.V
Subjects:
Bacteria
Ion exchange
Lipase
Medicine
Organic Chemistry
Pharmacy
Phenolphthalein
Plant Sciences
Pseudomonas aeruginosa
bacteria
activity
lipase
chromatography
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Description
Summary:A highly active and electrophoretically homogeneous enzyme with MM 31 kDa and specific lipase activity 1009 U/mg was isolated and purified from bacteria Pseudomonas aeruginosa A12 culture medium using ion-exchange and hydrophobic chromatography. The physicochemical properties of the isolated lipase were studied.
ISSN:0009-3130
1573-8388
DOI:10.1007/s10600-019-02909-y