Expression of the mouse interleukin-2 receptor gamma chain in various cell populations of the thymus and spleen

• Published in: European journal of immunology Vol. 24; no. 9; p. 2026
• Main Authors: Kondo, M, Ohashi, Y, Tada, K, Nakamura, M, Sugamura, K
• Format: Journal Article
• Language: English
• Published: Germany 01-09-1994
• Subjects: Animals; Antibodies, Monoclonal; CD4 Antigens - immunology; CD8 Antigens - immunology; Female; Fluorescent Antibody Technique; Lymphocytes - immunology; Mice; Mice, Inbred C57BL; Receptors, Interleukin-2 - biosynthesis; Receptors, Interleukin-2 - chemistry; Spleen - cytology; Spleen - immunology; Thymus Gland - cytology; Thymus Gland - embryology; Thymus Gland - growth & development; Thymus Gland - immunology
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830240914

Expression of the gamma chain, which is shared among functional receptor complexes for interleukin (IL)-2, IL-4 and IL-7, was examined with hematopoietic cells in mouse thymus and spleen by flow cytometry. The gamma chain was expressed in cell populations from the spleen. Stimulation with concanavalin A and lipopolysaccharide caused fluctuation in expression of the gamma chain in T and B cells, respectively. T lineage cells developing in the adult thymus expressed the gamma chain. Fetal thymus at day 15 contained mostly immature thymocytes, which also expressed the gamma chain. These results demonstrate that the gamma chains is widely expressed in T lineage cells, probably indicating that the gamma chain plays a role not only in the proliferation of mature hematopoietic cells but also in the development of immature cells through signal transduction as a common receptor subunit for multiple cytokines.