Analysis of the mechanism of chloramphenicol acetyltransferase by steady-state kinetics. Evidence for a ternary-complex mechanism

The mechanism of the enzymic reaction responsible for chloramphenicol resistance in bacteria was examined by steady-state kinetic methods. The forward reaction catalysed by chloramphenicol acetyltransferase leads to inactivation of the antibiotic. Use of alternative acyl donors and acceptors, as wel...

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Bibliographic Details

Published in:	Biochemical journal Vol. 223; no. 1; pp. 211 - 220
Main Authors:	Kleanthous, C, Shaw, W V
Format:	Journal Article
Language:	English
Published:	England 01-10-1984
Subjects:	Acetyl Coenzyme A - metabolism Acetylation Acetyltransferases - metabolism Chloramphenicol - metabolism Chloramphenicol O-Acetyltransferase Coenzyme A - metabolism Kinetics Macromolecular Substances Models, Chemical
Online Access:	Get full text
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