Properties of Some Norvaline-resistant Mutants of Bacillus subtilis

• Published in: Journal of general microbiology Vol. 88; no. 2; pp. 289 - 294
• Main Authors: HOLTZCLAW, W. D, CHAPMAN, LINDA F
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-06-1975
• Subjects: 2-Isopropylmalate Synthase - metabolism; Acetolactate Synthase - metabolism; Bacillus subtilis - enzymology; Bacillus subtilis - metabolism; Chromosome Mapping; Culture Media; Drug Resistance, Microbial; Enzyme Repression; Genes; Genetic Linkage; Glutamates - biosynthesis; Hydro-Lyases - metabolism; Isomerism; Leucine - biosynthesis; Mutation; Operon; Threonine Dehydratase - metabolism; Transaminases - metabolism; Transformation, Genetic; Valine - biosynthesis; Valine - pharmacology
• ISSN: 0022-1287
• DOI: 10.1099/00221287-88-2-289

Division of Biological Sciences, University of Missouri, Columbia, Missouri 65201, U.S.A.
ABSTRACT
SUMMARY: DL-Norvaline inhibits growth of wild-type Bacillus subtilis. A number of mutants resistant to growth inhibition by this analogue were isolated and studied. Cross-feeding experiments and paper chromatography of culture supernatants indicated that the mutants excreted leucine and possibly valine and glutamate. Enzymic analysis indicated that the mutants were derepressed for acetohydroxy-acid synthetase and -isopropylmalate synthetase; however, no derepression of threonine deaminase, dihydroxyacid dehydrase or transaminase B was observed.