Contribution of SELP and PSGL-1 genotypes and haplotypes to the presence of coronary heart disease in Tunisians

• Published in: Molecular biology reports Vol. 38; no. 1; pp. 495 - 501
• Main Authors: Ghazouani, Lakhdar, Abboud, Nesrine, Khalifa, Sonia Ben Hadj, Perret, Claire, Nicaud, Viviane, Almawi, Wassim Youssef, Cambien, François, Mahjoub, Touhami
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2011; Springer Nature B.V
• Subjects: Adult; Alleles; Animal Anatomy; Animal Biochemistry; Biomedical and Life Sciences; Cardiovascular disease; Case-Control Studies; Coronary Disease - genetics; Coronary vessels; Female; Gene Frequency - genetics; Genetic Predisposition to Disease; Genotype & phenotype; Haplotypes - genetics; Histology; Humans; Life Sciences; Linkage Disequilibrium - genetics; Male; Membrane Glycoproteins - genetics; Middle Aged; Minisatellite Repeats - genetics; Morphology; Odds Ratio; P-Selectin - genetics; Polymorphism, Single Nucleotide - genetics; Tunisia; Tunisia; Genetics; Adhesion molecules; Coronary heart disease; Atherosclerosis
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-010-0133-z

P-selectin (SELP) and its counter-receptor, P-selectin glycoprotein ligand-1 (PSGL-1), play key role in the transient attachment of leukocytes to endothelial cells predisposing to coronary heart disease (CHD). In the current report, 293 angiographically proven CHD patients and 327 age, gender, and race-matched controls were included. Our aim was to evaluate the contribution to CHD of the following SNPs: C-2123G, G-1969A and T715P in SELP, Met62Ile and the VNTR variants in
PSGL
-
1
gene in a North African population from Tunisia. While there were no significant differences in the distribution of SELP or PSGL-1 alleles or genotypes between patients and controls, a trend for a significant association of the C-2123G genotypes distribution with incident CHD was observed (
P
 = 0.06). Assuming an additive model of transmission, the risk was 74% higher among subjects carrying the GG genotypes in comparison to those carrying the CC genotype (OR = 1.74 [1.01–2.98],
P
 = 0.04) and 80% higher in the recessive model (OR = 1.80 [1.08–3.01],
P
 = 0.02). Haplotype analysis did not identify any specific SELP or PSGL-1 haplotypes to be associated with CHD. The present study demonstrated no evidence of association between individual SELP or PSGL-1 SNPs or haplotypes with incident CHD. However, this study replicates absence of association of the mostly studied SNP, T715P, previously reported in individuals with African origin.