Liquid-liquid extraction of an extracellular alkaline protease from fermentation broth using aqueous two-phase and reversed micelles systems
The study of recovery of an extracellular alkaline protease from fermentation broth produced by Norcadiopsis sp, was carried out with liquid-liquid extraction through sodium di-(2-ethylhexyl) sulphosuccinate/isooctane reversed micelles systems and aqueous two-phase systems (polyethylene glycol/potas...
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| Published in: | World journal of microbiology & biotechnology Vol. 21; no. 5; pp. 655 - 659 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Springer
01-07-2005
Springer Nature B.V |
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| Abstract | The study of recovery of an extracellular alkaline protease from fermentation broth produced by Norcadiopsis sp, was carried out with liquid-liquid extraction through sodium di-(2-ethylhexyl) sulphosuccinate/isooctane reversed micelles systems and aqueous two-phase systems (polyethylene glycol/potassium phosphate). The best conditions for extraction and back-extraction with the reversed micelles system was obtained at pH 9.0 and pH 5.0, respectively, showing a yield of protein of 6.16%, a specific activity of 4.10U/ml and a purification factor of 1.80. The studies using aqueous two-phase systems of polyethylene glycol/potassium phosphate at pH 10.0 showed purification factors of 2 and 5, and protein yield of 11 and 4%, respectively, for polyethylene glycol 550/potassium phosphate and polyethylene glycol 8000/potassium phosphate. The results indicate that the aqueous two-phase systems are more attractive as a first step in the isolation and purification processes.[PUBLICATION ABSTRACT] |
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| AbstractList | The study of recovery of an extracellular alkaline protease from fermentation broth produced by Norcadiopsis sp, was carried out with liquid-liquid extraction through sodium di-(2-ethylhexyl) sulphosuccinate/isooctane reversed micelles systems and aqueous two-phase systems (polyethylene glycol/potassium phosphate). The best conditions for extraction and back-extraction with the reversed micelles system was obtained at pH 9.0 and pH 5.0, respectively, showing a yield of protein of 6.16%, a specific activity of 4.10 U/ml and a purification factor of 1.80. The studies using aqueous two-phase systems of polyethylene glycol/potassium phosphate at pH 10.0 showed purification factors of 2 and 5, and protein yield of 11 and 4%, respectively, for polyethylene glycol 550/potassium phosphate and polyethylene glycol 8000/potassium phosphate. The results indicate that the aqueous two-phase systems are more attractive as a first step in the isolation and purification processes. The study of recovery of an extracellular alkaline protease from fermentation broth produced by Norcadiopsis sp, was carried out with liquid-liquid extraction through sodium di-(2-ethylhexyl) sulphosuccinate/isooctane reversed micelles systems and aqueous two-phase systems (polyethylene glycol/potassium phosphate). The best conditions for extraction and back-extraction with the reversed micelles system was obtained at pH 9.0 and pH 5.0, respectively, showing a yield of protein of 6.16%, a specific activity of 4.10U/ml and a purification factor of 1.80. The studies using aqueous two-phase systems of polyethylene glycol/potassium phosphate at pH 10.0 showed purification factors of 2 and 5, and protein yield of 11 and 4%, respectively, for polyethylene glycol 550/potassium phosphate and polyethylene glycol 8000/potassium phosphate. The results indicate that the aqueous two-phase systems are more attractive as a first step in the isolation and purification processes.[PUBLICATION ABSTRACT] |
| Author | MOREIRA, K. A SILVA, M. P. C LIMA-FILHO, J. L PORTO, T. S PORTO, A. L. F CARNEIRO-DA-CUNHA, M. G MONTEIRO, T. I. R |
| Author_xml | – sequence: 1 givenname: T. S surname: PORTO fullname: PORTO, T. S organization: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670- 420, Recife, PE, Brazil – sequence: 2 givenname: T. I. R surname: MONTEIRO fullname: MONTEIRO, T. I. R organization: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670- 420, Recife, PE, Brazil – sequence: 3 givenname: K. A surname: MOREIRA fullname: MOREIRA, K. A organization: Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco-UFPE, Brazil – sequence: 4 givenname: J. L surname: LIMA-FILHO fullname: LIMA-FILHO, J. L organization: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670- 420, Recife, PE, Brazil – sequence: 5 givenname: M. P. C surname: SILVA fullname: SILVA, M. P. C organization: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670- 420, Recife, PE, Brazil – sequence: 6 givenname: A. L. F surname: PORTO fullname: PORTO, A. L. F organization: Laboratório de Imunopatologia Keizo Asami-LIKA, Universidade Federal de Pernambuco-UFPE, Brazil – sequence: 7 givenname: M. G surname: CARNEIRO-DA-CUNHA fullname: CARNEIRO-DA-CUNHA, M. G organization: Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Prof. Moraes Rego s/n, CEP 50.670- 420, Recife, PE, Brazil |
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| Cites_doi | 10.1016/S1369-703X(02)00181-X 10.1016/S0960-8524(03)00033-6 10.1016/0022-2836(73)90083-1 10.1016/S0144-8617(01)00376-9 10.1002/(SICI)1097-4660(199705)69:1<77::AID-JCTB666>3.0.CO;2-V 10.1016/0141-0229(95)00045-3 10.1016/0378-4347(95)00419-X 10.1016/S0378-4347(97)00201-6 10.1016/S1570-0232(02)00409-9 10.1111/j.1745-4514.2001.tb00721.x 10.1016/S0032-9592(98)00076-4 10.1016/0141-0229(91)90184-C 10.1016/0003-2697(85)90442-7 10.1002/bit.260460210 10.1002/btpr.5420040405 10.1021/bp00024a012 10.1016/S0032-9592(00)00212-0 10.1002/bit.260381107 10.1007/BF02788066 10.1016/S0032-9592(02)00312-6 10.1002/(SICI)1097-0290(19990605)63:5<583::AID-BIT8>3.0.CO;2-U 10.1023/A:1015557802326 |
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| Keywords | Alkaline proteinase Enzyme Liquid liquid extraction liquid-liquid extraction proteases Fermentation Extracellular Peptidases Biphasic system Hydrolases reversed micelles Aqueous two-phase systems Reverse micelle |
| Language | English |
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| SubjectTerms | Biological and medical sciences Biotechnology Enzyme engineering Fermentation Fundamental and applied biological sciences. Psychology Improved methods for extraction and purification of enzymes Methods. Procedures. Technologies Polyethylene glycol Potassium Proteases Studies |
| Title | Liquid-liquid extraction of an extracellular alkaline protease from fermentation broth using aqueous two-phase and reversed micelles systems |
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