Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus： karyotype, allozyme and RAPD analyses

• Published in: Chinese journal of oceanology and limnology Vol. 27; no. 2; pp. 317 - 323
• Main Author: 尤锋 王伟 徐冬冬 朱香萍 倪静 吴志昊 徐永立 王新成 张培军
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01-05-2009; Springer Nature B.V
• Subjects: Allozymes; Artificial insemination; Biological fertilization; Biology; Earth and Environmental Science; Earth Sciences; Fish; Flounder; Genetic distance; Genetic diversity; Genetic markers; Genetic variation; Heterozygosity; Hybridization; Hybrids; Isoenzymes; Kareius bicoloratus; Karyotypes; Marine biology; Marine fishes; Metamorphosis; Nucleotide sequence; Oceanography; Offspring; Paralichthys olivaceus; Parental stocks (fishery); Primers; Progeny; Random amplified polymorphic DNA; RAPD分析; Spermatozoa; Stone; 杂交后代; 染色体核型; 橄榄树; artificial hybridization; karyotype; Temminck et Schlegel; RAPD; Basilewsky; allozyme
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-009-9134-y

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.