Infrequent loss of heterozygosity of the major tumour suppressor genes in Indian oral cancers

• Published in: International journal of oral and maxillofacial surgery Vol. 31; no. 4; pp. 414 - 418
• Main Authors: Kannan, S., Yokozaki, H., Jayasree, K., Sebastian, P., Mathews, A., Abraham, E.K., Nair, M.K., Tahara, E.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01-08-2002; Elsevier
• Subjects: Adult; Aged; apc gene; Biological and medical sciences; Carcinoma, Squamous Cell - epidemiology; Carcinoma, Squamous Cell - genetics; Case-Control Studies; Dentistry; Female; Gene Frequency; Genes, APC - physiology; Genes, p53 - genetics; Genes, Retinoblastoma - genetics; Genes, Tumor Suppressor - physiology; Humans; India - epidemiology; Loss of Heterozygosity; Male; Medical sciences; Middle Aged; Minisatellite Repeats; Molecular Epidemiology; Mouth Neoplasms - epidemiology; Mouth Neoplasms - genetics; oral squamous cell carcinoma; Otorhinolaryngology. Stomatology; p53; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Tropical medicine; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Asia; India; rb; oral squamous cell carcinoma; loss of heterozygosity; p53; apc gene; Human; Squamous cell carcinoma; Stomatology; Etiopathogenesis; Exploration; Malignant tumor; Polymerase chain reaction; Oral cavity; Microsatellite DNA; Loss of heterozygosity; Genetics; Adult; Oral cavity disease; Molecular biology; Tumor suppressor gene
• ISSN: 0901-5027; 1399-0020
• DOI: 10.1054/ijom.2001.0155

The loss of heterozygosity (LOH) in tumour suppressor gene loci such as p53, retinoblastoma (rb) and adenomatous polyposis coli (apc) were analyzed in oral cancer tissues with matched controls by employing polymerase chain reaction based/restriction fragment length polymorphism (PCR-RFLP), variable number of tandem repeats (PCR-VNTR) analysis and microsatellite assay. The PCR-RFLP analysis showed an infrequent LOH in rb (17%), p53 (11%) and apc (10%) loci in these cases. The microsatellite assay also revealed only a low frequency of LOH in the microsatellite markers such as TP53 (25%), D5S505 (10%) and D3S1067 (0%) in the same samples. In contrast to the present study, similar studies from Western countries have reported a high frequency of LOH in p53, rb and apc genes in oral cancer tissues. The present preliminary study indicates that the gene aberration by LOH may be an insignificant mechanism in Indian oral cancers with respect to the tumour suppressor genes examined.