Acute toxicity and cytotoxicity evaluation of Dendrobium moniliforme aqueous extract in vivo and in vitro

• Published in: Laboratory animal research Vol. 32; no. 3; pp. 144 - 150
• Main Authors: Lee, Mu-Jin, Jung, Ho-Kyung, Kim, Min-Suk, Jang, Ji-Hun, Sim, Mi-Ok, Kim, Tea-Mook, Park, Ho, Ahn, Byung-Kwan, Cho, Hyun-Woo, Cho, Jung-Hee, Jung, Won-Seok, Kim, Jong-Choon
• Format: Journal Article
• Language: English
• Published: England Korean Association for Laboratory Animal Science 01-09-2016; BMC; 한국실험동물학회
• Subjects: acute toxicity; cytotoxicity; Dendrobium moniliforme; minimal lethal dose (LD10); Original; 수의학; Dendrobium moniliforme; minimal lethal dose (LD10); acute toxicity; cytotoxicity
• ISSN: 1738-6055; 2233-7660
• DOI: 10.5625/lar.2016.32.3.144

(L.) Sw., an herb of the
family, has long been used in traditional medicine to strengthen bones, nourish the stomach, and promote the production of bodily fluid. Recently, polysaccharides isolated from
have been used in functional foods and nutraceutical products. A traditional method to process
is to soak fresh stems in an ethanol solution, which is the most important factor to ensure high yields of aqueous-extractable polysaccharides. The present study was carried out to investigate the potential acute toxicity of
aqueous extract (DMAE), by a single oral dose in Sprague-Dawley rats. The test article was orally administered once by gavage to male and female rats at doses of 0, 2,500, and 5,000 mg/kg body weight (n=5 male and female rats for each dose). Throughout the study period, no treatment-related deaths were observed and no adverse effects were noted in clinical signs, body weight, food consumption, serum biochemistry, organ weight, or gross findings at any dose tested. The results show that a single oral administration of DMAE did not induce any toxic effects at a dose below 5,000 mg/kg in rats, and the minimal lethal dose was considered to be over 5,000 mg/kg body weight for both sexes. With respect to cytotoxicity, the cell viability of human embryonic kidney (HEK293) cells was less than 50% when the cells were treated with 10 mg/mL aqueous extract for 24 h.