Intracellular Targeting of Calmodulin mRNAs in Primary Hippocampal Cells

We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was...

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Bibliographic Details
Published in:	The journal of histochemistry and cytochemistry Vol. 51; no. 4; pp. 541 - 544
Main Authors:	Kortvely, Elod, Varszegi, Szilvia, Palfi, Arpad, Gulya, Karoly
Format:	Journal Article
Language:	English
Published:	Los Angeles, CA Histochemical Soc 01-04-2003 SAGE Publications
Subjects:	Animals Calmodulin - genetics Calmodulin - metabolism Cells, Cultured Embryo, Mammalian Hippocampus - cytology Hippocampus - metabolism Immunohistochemistry In Situ Hybridization Rats RNA, Messenger - metabolism mRNA localization hippocampal cell culture calmodulin dendritic intracellular S100 immunocytochemistry
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Summary:	We investigated the intracellular distribution of the mRNAs corresponding to the three non-allelic CaM genes in cultured hippocampal cells by in situ hybridization with digoxigenin-labeled gene-specific riboprobes. In neurons the perikaryon was heavily stained and strong dendritic mRNA targeting was detected for all three CaM genes. The color labeling exhibited a punctate distribution, suggesting that CaM mRNAs are transported in RNA granules. Immunocytochemistry for S100 demonstrated that glial cells express CaM mRNAs at a very low level. A minority of the cultured cells were negative for either labeling.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-1554 1551-5044
DOI:	10.1177/002215540305100416