In vivo production of neuraminidase by Pasteurella multocida A:3 in goats after transthoracic challenge

• Published in: Current microbiology Vol. 33; no. 4; pp. 266 - 269
• Main Authors: Straus, D.C. (Texas Tech University Health Sciences Center, Lubbock, TX.), Cooley, J.D, Purdy, C.W
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-10-1996
• Subjects: Animals; Antibodies, Bacterial - analysis; ANTICORPS; ANTICUERPOS; Bacteriology; Biological and medical sciences; BIOSINTESIS; BIOSYNTHESE; Biotechnology; CAPRIN; CAPRINOS; Female; Fundamental and applied biological sciences. Psychology; GLICOSIDASAS; GLYCOSIDASE; Goats; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; Male; Microbiology; Neuraminidase - biosynthesis; Neuraminidase - immunology; NEUTRALISATION; NEUTRALIZACION; Neutralization Tests; Pasteurella Infections - enzymology; Pasteurella Infections - immunology; Pasteurella Infections - microbiology; PASTEURELLA MULTOCIDA; PATHOGENESE; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; PATOGENESIS; Enzyme; Respiratory disease; Host agent relation; Biosynthesis; Pasteurellosis; Pasteurella multocida; Infection; Pasteurellaceae; In vivo; Vertebrata; Experimental disease; Mammalia; Glycosidases; Animal; Exo-α-sialidase; Bacteriosis; Bacteria; Hydrolases; Goat; Artiodactyla; Veterinary; O-Glycosidases; Ungulata
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s002849900111

Six goats were injected transthoracically with live Pasteurella multocida A:3 to examine if an extracellular enzyme, neuraminidase, was produced in vivo during infection with this organism. The principal group of goats (n = 6) each received 1 ml of live 7.5 X 10(4) cfu of P. multocida mixed with polyacrylate beads transthoracically in the left lung on day 0 and 1 ml of live P. multocida (2.2 X 10(8) cfu) mixed with polyacrylate beads transthoracically in the left lung on day 22. Six coats were used as negative controls and received 0.3 g of polyacrylate beads subcutaneously in the right flank on days 0 and 22. Serum was obtained from all animals on days 0, 7, 14, 22, 29, and 36. Preimmune sera from all animal showed no detectable antibody to P. multocida A:3 neuraminidase in an enzyme neutralization assay. None of the sera from the negative control animals demonstrated a significant antibody titer against the P. multocida A:3 neuraminidase. On day 36, serum samples from the six infected animals possessed complete enzyme-neutralizing activity. Anti-neuraminidase antibody could be detected as early as day 14 in the infected animals. These data show that neuraminidase is produced in vivo during an active P. multocida A:3 lobar infection