Lack of Clinical Usefulness of Das-1 Monoclonal Antibody and Mucin Expression as Risk Markers of Gastric Carcinoma in Patients With Gastric Intestinal Metaplasia

• Published in: American journal of clinical pathology Vol. 131; no. 1; pp. 99 - 105
• Main Authors: FORNE, Montserrat, FERNANCLEZ-BANARES, Fernanda, SALAS, Antonio, VIVER, Josep M, GONZALEZ-MINGUEZ, Clarisa, CASALOTS, Jaume, POBLET-MAS, Nuria, GARCIA-GIL, L. Jesus, ESTEVE, Maria, ROSINACH, Mercè, ESPINOS, Jorge, LORAS, Carme
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Society of Clinical Pathologists 2009
• Subjects: Adult; Aged; Antibodies; Antibodies, Monoclonal; Biological and medical sciences; Gastritis - pathology; Gastroenterology. Liver. Pancreas. Abdomen; Helicobacter pylori; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Metaplasia - pathology; Middle Aged; Mucins - biosynthesis; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Risk; Stomach - pathology; Stomach Neoplasms - etiology; Stomach Neoplasms - pathology; Stomach Ulcer - pathology; Stomach Ulcer - physiopathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Spirillaceae; Biological marker; Gastric intestinal metaplasia; Risk; Monoclonal antibody; Stomach cancer; Stomach carcinoma; Helicobacter pylori; Bacteria; Gastric disease; Human; Premalignant lesion; Spirillales; Biological indicator; Gastric adenocarcinoma; Malignant tumor; Stomach adenocarcinoma; Infection; Anatomic pathology; Mucin; Das-1; Risk factor; Mucins; Bacteriosis; Digestive diseases; Cancer
• ISSN: 0002-9173; 1943-7722
• DOI: 10.1309/AJCPNP7BK3KUUOJL

Our aim was to evaluate the usefulness of the monoclonal antibody Das-1 as a premalignant marker of gastric intestinal metaplasia (GIM) associated with gastric cancer and its association with mucin expression. We evaluated Das-1 and mucin expression in 4 groups: 1 (n = 50), gastric carcinoma, paired samples of the cancer area and GIM away from the tumor; 2 (n = 25), gastric or duodenal ulcer with Helicobacter pylori infection with GIM and chronic gastritis; 3 (n = 25),H pylori- autoimmune chronic atrophic gastritis with GIM; and 4 (n = 25),H pylori- chronic gastritis without GIM. Das-1 immunostaining was observed in 20 (40%) of 50 cases in cancer areas. The expression of Das-1 in GIM from group 1 cases away from the cancer area was different from that in GIM from nontumor cases (groups 2 and 3): 13 (26%) of 50 vs 2 (8%) and 0 (0%) of 25 (P = .004). There was no association between Das-1 and mucin expression. Das-1 expression was associated with GIM from patients with gastric cancer. However, this relation was weaker than previously reported, precluding clinical usefulness as a premalignant marker of GIM.