Development of slide ELISA (SELISA) for detection of four poultry viral pathogens by direct heat fixation of viruses on glass slides

• Published in: Journal of virological methods Vol. 209; pp. 76 - 81
• Main Authors: Desingu, P.A., Singh, S.D., Dhama, K., Vinodh Kumar, O.R., Singh, R., Singh, R.K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-12-2014
• Subjects: Animals; DNA Viruses - isolation & purification; Enzyme-Linked Immunosorbent Assay - methods; Glass; Hot Temperature; Microscopy; Poultry; Poultry Diseases - diagnosis; Poultry Diseases - virology; Poultry viruses; RNA Viruses - isolation & purification; Semi quantitation; Slide ELISA; Specimen Handling - methods; Veterinary Medicine - methods; Virology - methods; Virus Diseases - diagnosis; Virus Diseases - veterinary; Virus Diseases - virology; Slide ELISA; Poultry viruses; Semi quantitation
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2014.08.010

•To detect poultry viral agents by SELISA.•The positive signals for viral aggregates were detected under light microscope.
The development of an easy and simpler method of slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of four economically important poultry viruses viz., Newcastle disease virus (NDV), infectious bronchitis virus (IBV), infectious bursal disease virus (IBDV) and egg drop syndrome 76 virus (EDS 76) and the use of SELISA for semi quantitation of NDV are described. The positive signals for viral aggregates were detected under light microscope. This is the first report regarding the development of SELISA based on heat fixation for the diagnosis of viral pathogens.