Layer-by-layer assembly of DNA-dye complex films

• Published in: Thin solid films Vol. 409; no. 2; pp. 227 - 232
• Main Authors: Chen, Xiaodong, Lang, Jie, Liu, Minghua
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 30-04-2002; Elsevier Science
• Subjects: Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; DNA; Dye; Exact sciences and technology; Layer-by-layer; Liquid phase epitaxy; deposition from liquid phases (melts, solutions, and surface layers on liquids); Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties; Materials science; Methods of deposition of films and coatings; film growth and epitaxy; Multilayers; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation; Optical properties of specific thin films; Optical properties of specific thin films, surfaces, and low-dimensional structures; Physics; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Multilayers; Dye; DNA; Layer-by-layer; Pentacyclic compound; Fluorescence spectrum; Atomic layer method; Organic cation; Organic dye; Nitrogen heterocycle; Iminium compounds; Sulfonates; Experimental study; Macrocycle; Thin films; Organic anion; Lifetime; Aqueous solutions; Six membered ring; Ultraviolet visible spectrum; Absorption spectra; Deposition; Growth from solution; Organic compounds; Adducts
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/S0040-6090(02)00045-7

Complex films of 5,10,15,20-tetrakis(4-
N-methylpyridyl)porphine tetra(
p-toluenesulfonate) (TMPyP) and deoxyribonucleic acid (DNA) were formed by sequential deposition of TMPyP and DNA from aqueous solution onto quartz substrates. The deposition was monitored by absorption spectroscopy. It revealed that the DNA-TMPyP complex film is uniformly assembled layer-by-layer and the net charge of the dye is important in forming the DNA-dye film. The resulting DNA-dye complex multilayer films were characterized by UV-vis spectroscopy, fluorescence spectroscopy and fluorescence lifetime measurements. It has been found that TMPyP can either bind to the DNA electrostatically or intercalate into the base pairs. The relative ratio of the TMPyP intercalating into the DNA in the film is smaller than that in solution.