Comparison of the long-term effects on rabbit bone defects between Tetrabone® and β-tricalcium phosphate granules implantation

• Published in: Journal of artificial organs Vol. 17; no. 4; pp. 344 - 351
• Main Authors: Choi, Sungjin, Liu, I-Li, Yamamoto, Kenichi, Honnami, Muneki, Ohba, Shinsuke, Echigo, Ryosuke, Sakai, Takamasa, Igawa, Kazuyo, Suzuki, Shigeki, Nishimura, Ryouhei, Chung, Ung-il, Sasaki, Nobuo, Mochizuki, Manabu
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01-12-2014
• Subjects: Animals; Biocompatible Materials; Biomedical Engineering and Bioengineering; Bone Regeneration - physiology; Bone Substitutes; Calcium Phosphates; Cardiac Surgery; Femur - physiology; Medicine; Medicine & Public Health; Nephrology; Original Article; Osteogenesis - physiology; Rabbits; Rabbit; Artificial bones; β-TCP granules; OCP; Tetrabone
• ISSN: 1434-7229; 1619-0904
• DOI: 10.1007/s10047-014-0778-9

Tetrabone
®
is a newly developed granular artificial bone. The 1-mm Tetrabone
®
has a four-legged structure. In this study, the long-term effect of implanting Tetrabone
®
or β-TCP granules in rabbit femoral cylindrical defects was evaluated. The rabbits were euthanized at 4, 13, and 26 weeks after implantation. Micro-CT was conducted to evaluate the residual material volume and the non-osseous tissue volume. New bone tissue areas were measured by histological analysis. Micro-CT imaging showed that the residual material volume in the β-TCP group had decreased significantly at 4 weeks after implantation (
P
 < 0.05) and that the β-TCP granules had nearly disappeared at 26 weeks after implantation. In the Tetrabone
®
group, it did not significantly change until 13 weeks after implantation; it then continued to decrease slightly until 26 weeks after implantation. The non-osseous volume increased in the β-TCP group, whereas that of the Tetrabone
®
group decreased (
P
 < 0.05). Histological examination showed that the new bone areas were significantly greater in the Tetrabone
®
group than in the β-TCP group at 13 and 26 weeks. In conclusion, resorption of β-TCP granules occurs before sufficient bone formation, thereby allowing non-osseous tissue invasion. Tetrabone
®
resorption progressed slowly while the new bone tissues were formed, thus allowing better healing. Tetrabone
®
showed better osteoconductivity, whereas the β-TCP granules lost their function over a long duration. These results may be caused by the differences in the absorption rate of the granules, intergranular pore structure, and crystallinity of each granule.