Development of recombinant nonstructural 1 protein based indirect enzyme linked immunosorbent assay for sero-surveillance of Japanese encephalitis in swine

•An indirect ELISA using recombinant NS1 protein to detect anti-JEV IgG antibody in swine.•Developed ELISA validated in 10 different laboratories revealing excellent reproducibility.•Screening of 3628 swine Serum samples revealed sero-positivity of 32.22% against JE virus.•Developed ELISA can be use...

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Published in:	Journal of virological methods Vol. 272; p. 113705
Main Authors:	Dhanze, H., Bhilegaonkar, K.N., Rawat, S., Chethan Kumar, H.B., Kumar, A., Gulati, B.R., Mishra, B.P., Singh, R.K.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-10-2019
Subjects:	Animals ELISA Encephalitis Virus, Japanese - genetics Encephalitis Virus, Japanese - isolation & purification Encephalitis, Japanese - diagnosis Encephalitis, Japanese - immunology Encephalitis, Japanese - veterinary Encephalitis, Japanese - virology Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Immunoglobulin G India Japanese encephalitis Neutralization Tests - methods Recombinant Proteins - genetics Recombinant Proteins - immunology Surveillance Swine Swine Diseases - diagnosis Swine Diseases - virology Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - immunology India Surveillance Swine ELISA Japanese encephalitis
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Abstract	•An indirect ELISA using recombinant NS1 protein to detect anti-JEV IgG antibody in swine.•Developed ELISA validated in 10 different laboratories revealing excellent reproducibility.•Screening of 3628 swine Serum samples revealed sero-positivity of 32.22% against JE virus.•Developed ELISA can be used in surveillance programs for detection of JEV activity in swine population thereby aiding in prevention of outbreaks in humans. Japanese encephalitis virus (JEV) causes severe neurological disease in humans, especially among children. The disease is endemic in several South Asian countries including India. Swine play a major role as amplifier host for JEV and act as a source of infection to humans through mosquito bite. Early detection of either virus or antibodies in swine will aid to undertake control measures to prevent virus spread to humans. Swine seldom show symptoms of JEV infection and the viraemic phase lasts for a short period of 3 to 4 days indicating the potential of detection of antibodies, which remain for relatively longer period, as a suitable alternative. Cost effective and sensitive assays for the detection of JEV antibodies in swine are not available indigenously. Hence, we have developed a recombinant nonstructural protein 1 (rNS1) based enzyme linked immunosorbent assay for the detection of IgG antibodies against JEV in swine. The test is robust, highly sensitive (91%), specific (97%), reproducible and affordable. Field validation of the assay was done by screening 3628 swine Serum samples collected from different parts of India. The overall sero-positivity was found to be 32.22%. The developed ELISA can be readily incorporated into surveillance programs for detection of Japanese encephalitis virus activity in swine population thereby aiding in prediction of outbreaks in humans.
AbstractList	Japanese encephalitis virus (JEV) causes severe neurological disease in humans, especially among children. The disease is endemic in several South Asian countries including India. Swine play a major role as amplifier host for JEV and act as a source of infection to humans through mosquito bite. Early detection of either virus or antibodies in swine will aid to undertake control measures to prevent virus spread to humans. Swine seldom show symptoms of JEV infection and the viraemic phase lasts for a short period of 3 to 4 days indicating the potential of detection of antibodies, which remain for relatively longer period, as a suitable alternative. Cost effective and sensitive assays for the detection of JEV antibodies in swine are not available indigenously. Hence, we have developed a recombinant nonstructural protein 1 (rNS1) based enzyme linked immunosorbent assay for the detection of IgG antibodies against JEV in swine. The test is robust, highly sensitive (91%), specific (97%), reproducible and affordable. Field validation of the assay was done by screening 3628 swine Serum samples collected from different parts of India. The overall sero-positivity was found to be 32.22%. The developed ELISA can be readily incorporated into surveillance programs for detection of Japanese encephalitis virus activity in swine population thereby aiding in prediction of outbreaks in humans. •An indirect ELISA using recombinant NS1 protein to detect anti-JEV IgG antibody in swine.•Developed ELISA validated in 10 different laboratories revealing excellent reproducibility.•Screening of 3628 swine Serum samples revealed sero-positivity of 32.22% against JE virus.•Developed ELISA can be used in surveillance programs for detection of JEV activity in swine population thereby aiding in prevention of outbreaks in humans. Japanese encephalitis virus (JEV) causes severe neurological disease in humans, especially among children. The disease is endemic in several South Asian countries including India. Swine play a major role as amplifier host for JEV and act as a source of infection to humans through mosquito bite. Early detection of either virus or antibodies in swine will aid to undertake control measures to prevent virus spread to humans. Swine seldom show symptoms of JEV infection and the viraemic phase lasts for a short period of 3 to 4 days indicating the potential of detection of antibodies, which remain for relatively longer period, as a suitable alternative. Cost effective and sensitive assays for the detection of JEV antibodies in swine are not available indigenously. Hence, we have developed a recombinant nonstructural protein 1 (rNS1) based enzyme linked immunosorbent assay for the detection of IgG antibodies against JEV in swine. The test is robust, highly sensitive (91%), specific (97%), reproducible and affordable. Field validation of the assay was done by screening 3628 swine Serum samples collected from different parts of India. The overall sero-positivity was found to be 32.22%. The developed ELISA can be readily incorporated into surveillance programs for detection of Japanese encephalitis virus activity in swine population thereby aiding in prediction of outbreaks in humans.
ArticleNumber	113705
Author	Singh, R.K. Gulati, B.R. Dhanze, H. Chethan Kumar, H.B. Rawat, S. Kumar, A. Mishra, B.P. Bhilegaonkar, K.N.
Author_xml	– sequence: 1 givenname: H. surname: Dhanze fullname: Dhanze, H. email: hdhanze@yahoo.co.in organization: ICAR- Indian Veterinary Research Institute, India – sequence: 2 givenname: K.N. surname: Bhilegaonkar fullname: Bhilegaonkar, K.N. organization: ICAR- Indian Veterinary Research Institute, India – sequence: 3 givenname: S. surname: Rawat fullname: Rawat, S. organization: Department of Veterinary Public Health & Epidemiology, SVPUA&T, Meerut, India – sequence: 4 givenname: H.B. surname: Chethan Kumar fullname: Chethan Kumar, H.B. organization: ICAR-Central Coastal Agricultural Research Institute, Goa, India – sequence: 5 givenname: A. surname: Kumar fullname: Kumar, A. organization: Indian Council of Agricultural Research, New Delhi, India – sequence: 6 givenname: B.R. surname: Gulati fullname: Gulati, B.R. organization: ICAR-National Research Centre on Equines, Hisar, India – sequence: 7 givenname: B.P. surname: Mishra fullname: Mishra, B.P. organization: ICAR- Indian Veterinary Research Institute, India – sequence: 8 givenname: R.K. surname: Singh fullname: Singh, R.K. organization: ICAR- Indian Veterinary Research Institute, India
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/31351167$$D View this record in MEDLINE/PubMed
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CitedBy_id	crossref_primary_10_1016_j_jim_2020_112848 crossref_primary_10_1016_j_ebiom_2021_103353 crossref_primary_10_1016_j_biologicals_2022_01_001 crossref_primary_10_1016_j_jevs_2021_103809 crossref_primary_10_4103_1995_7645_359788 crossref_primary_10_1111_zph_13123 crossref_primary_10_4103_ijmr_IJMR_740_21 crossref_primary_10_1016_j_pep_2019_105548 crossref_primary_10_1016_j_prevetmed_2020_104944 crossref_primary_10_1016_j_jviromet_2020_113876 crossref_primary_10_1016_j_biologicals_2019_09_003 crossref_primary_10_1016_j_pep_2021_106031 crossref_primary_10_1016_j_cimid_2024_102189 crossref_primary_10_4103_0972_9062_374041 crossref_primary_10_1016_j_jim_2024_113695
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Snippet	•An indirect ELISA using recombinant NS1 protein to detect anti-JEV IgG antibody in swine.•Developed ELISA validated in 10 different laboratories revealing... Japanese encephalitis virus (JEV) causes severe neurological disease in humans, especially among children. The disease is endemic in several South Asian...
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SubjectTerms	Animals ELISA Encephalitis Virus, Japanese - genetics Encephalitis Virus, Japanese - isolation & purification Encephalitis, Japanese - diagnosis Encephalitis, Japanese - immunology Encephalitis, Japanese - veterinary Encephalitis, Japanese - virology Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Immunoglobulin G India Japanese encephalitis Neutralization Tests - methods Recombinant Proteins - genetics Recombinant Proteins - immunology Surveillance Swine Swine Diseases - diagnosis Swine Diseases - virology Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - immunology
Title	Development of recombinant nonstructural 1 protein based indirect enzyme linked immunosorbent assay for sero-surveillance of Japanese encephalitis in swine
URI	https://dx.doi.org/10.1016/j.jviromet.2019.113705 https://www.ncbi.nlm.nih.gov/pubmed/31351167 https://search.proquest.com/docview/2265762362
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