Flow Cytometric Analysis of Chimerism in the Rat Tolerant to a Renal Allograft

Background.Chimerism, produced by the two-way migration of cells between graft and host, is a proposed mechanism by which tolerance occurs. The appearance of donor/recipient chimeras in tolerant ACI to Lewis rat heterotopic renal transplants was assessed in peripheral blood leukocytes using flow cyt...

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Published in:The Journal of surgical research Vol. 77; no. 2; pp. 179 - 186
Main Authors: Naar, J.D., Fisher, R.A., Saggi, B.H., Wakely, P.E., Tawes, J.W., Posner, M.P.
Format: Journal Article Conference Proceeding
Language:English
Published: New York, NY Elsevier Inc 01-07-1998
Elsevier
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Summary:Background.Chimerism, produced by the two-way migration of cells between graft and host, is a proposed mechanism by which tolerance occurs. The appearance of donor/recipient chimeras in tolerant ACI to Lewis rat heterotopic renal transplants was assessed in peripheral blood leukocytes using flow cytometry after staining with monoclonal antibodies. Materials and methods.ACI and Lewis rats were used as donor and recipient, respectively, after Rapamycin and Cyclosporin immunosuppression with or without donor blood or bone marrow transfusion. ACI and Lewis animals were also used for isograft and single-kidney controls. Animals were sacrificed at various time points after initial operation. Flow cytometry was performed on isolated peripheral blood leukocytes at sacrifice. Histologic and functional data were also obtained. The monoclonal antibody panel included RT1a(ACI, MHC I) combined with CD2, CD4, CD8, CD16, and CD25 or RT1a,c(bone marrow chimeras). Results.RT1a+, CD8+ cells were transiently present in the peripheral blood leukocytes of Lewis recipients with the exception of allogeneic bone marrow recipients. No significant number of RT1a+, CD16+ (“dendritic” cell-line) chimeras was seen. Veto cells (RT1a,c+) were transiently present in the bone marrow recipients, but they did not lead to improved outcome. Furthermore, no correlation was made between histologic tolerance and any of these donor-derived cells. Conclusion.Donor/recipient chimerism, and the veto cell phenomenon are not operational tolerance mechanisms in this stringent model of ACI to Lewis rat renal transplantation.
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ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.1998.5373