Heptyl oleate synthesis as useful tool to discriminate between lipases, proteases and other hydrolases in crude preparations

Heptyl oleate synthesis is a good test to compare crude lipases. Being a reaction selective for lipases, esterases and proteases are not active in this esterification. The heptyl oleate synthesis has been carried out with crude lipases and other hydrolases (such as proteases and esterases) from diff...

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Bibliographic Details
Published in:	Enzyme and microbial technology Vol. 31; no. 3; pp. 283 - 288
Main Authors:	Domı́nguez de Marı́a, Pablo, Martı́nez-Alzamora, Fernando, Moreno, Sergio Pérez, Valero, Francisco, Rúa, M.Luisa, Sánchez-Montero, José M, Sinisterra, José V, Alcántara, Andrés R
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Inc 02-08-2002 Elsevier Science
Subjects:	Biological and medical sciences Biotechnology Enzyme engineering Esterases Fundamental and applied biological sciences. Psychology Heptyl oleate synthesis Hydrolysis Lipases Methods. Procedures. Technologies Miscellaneous Hydrolysis Heptyl oleate synthesis Esterases Lipases Enzymatic synthesis Peptidases Enzymatic activity Enzyme Oleic acid Triacylglycerol lipase Hydrolases Fermentation Esterification Comparative study Carboxylic ester hydrolases
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