Non-enzymatic electrochemical detection of melamine in dairy products by using CuO decorated carbon nanotubes nanocomposites

• Published in: Food chemistry Vol. 445; p. 138792
• Main Authors: Algethami, Faisal K., Marwani, Hadi M., Raza, Nadeem, Asiri, Abdullah M., Rahman, Mohammed M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-07-2024
• Subjects: Animals; CuO-CNT composites; Dairy products; Electrochemical Techniques - methods; Electrodes; Fluorocarbon Polymers; Food safety; Glassy carbon electrode; Humans; Melamine; Milk; Nanocomposites - chemistry; Nanotubes, Carbon - chemistry; Oxides - chemistry; Quality control; Triazines; CuO-CNT composites; Glassy carbon electrode; Dairy products; Food safety; Melamine; Quality control
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2024.138792

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•CuO-CNT nanocomposites were prepared by facile wet-chemical technique.•Detection of melamine using a CuO-CNT NCs/Nafion/GCE.•It was found highly sensitive melamine (93.924 µA µM−1 m−2) detection.•The detection limit for melamine was found as low as 0.27 nM.•The proposed technique could detect melamine in dairy products with good recovery.
Melamine, a typical nitrogen enriched organic compound exhibiting great potential in the industrial sector, is exploited as an adulterant to inflate protein levels in dairy products, can pose serious threats to humans and therefore necessitates its swift detection and precise quantification at its first exposure. In this investigation, sensitive and reliable sensor probes were fabricated using CuO nanoparticles and its nanocomposites (NCs) with carbon nanotubes (CNTs), carbon black (CB), and graphene oxide (GO) to promptly quantify melamine in dairy products. The optical, morphological, and structural characteristics of the CuO-CNT NCs were achieved using diverse instrumental techniques including UV–visible spectroscopy, transmission electron microscopy, X- ray diffraction, Fourier-transform infrared spectroscopy, and X-ray photoelectron spectroscopy and etc. The fabrication of glassy carbon electrodes (GCE) was accomplished by coating CuO-CNT NCs through a binder (5 % nafion). These sensor probes demonstrated outstanding electrochemical sensor performance with CuO-CNT NCs/Nafion/GCE sensor probe in terms of very low limit of detection (0.27 nM), good linearity range (0.05–0.5 nM), and relatively high sensitivity (93.924 µA µM−1 m−2) for melamine under optimized experimental conditions. Furthermore, the performance of CuO-CNT NCs/Nafion/GCE coated sensor probes was practically validated for the selective melamine detection in the real sample analysis of commercially available milk brands, which revealed significant figures of merit in a very short response time of 10 s. From the results, it was concluded that the current study might be helpful in the development of an efficient commercial sensor based on ultra-sensitive transition metal oxides in the field of health care monitoring, food stuffs in a broader scale as well as food applications.