Effects of benzene metabolites on receptor-mediated phagocytosis and cytoskeletal integrity in mouse peritoneal macrophages

• Published in: Toxicology and applied pharmacology Vol. 126; no. 2; p. 214
• Main Authors: Manning, B W, Adams, D O, Lewis, J G
• Format: Journal Article
• Language: English
• Published: United States 01-06-1994
• Subjects: Actins - analysis; Animals; Benzene Derivatives - toxicity; Benzoquinones - toxicity; Catechols - toxicity; Cell Survival; Dose-Response Relationship, Drug; Female; Hydroquinones - toxicity; In Vitro Techniques; Macrophages, Peritoneal - cytology; Macrophages, Peritoneal - drug effects; Mice; Mice, Inbred C57BL; Phagocytosis - drug effects; Receptors, Fc - drug effects; Temperature; Time Factors
• ISSN: 0041-008X
• DOI: 10.1006/taap.1994.1110

Exposure to benzene can induce a number of hematotoxicities and decrease host resistance to microorganisms and tumors. Several studies have shown that metabolism of benzene to reactive intermediates is required for myelotoxicity. Since receptor-mediated phagocytosis by macrophages is an important host defense, we have examined the effects of benzene metabolites on receptor-mediated phagocytosis in cultured murine peritoneal macrophages. 1,4-Benzoquinone (BQ) was the most potent of the metabolites examined. Ten-minute exposures to a 12.5 microM concentration inhibited Fc and complement receptor-mediated phagocytosis by > or = 90%. Macrophage viability was largely unaffected by BQ treatment. Exposure to 50 and 100 microM 1,2,4-benzenetriol (BT) inhibited Fc receptor-mediated phagocytosis by 70 and 95%, respectively. Hydroquinone (HQ) elicited a major decrease (50%) only at 100 microM. The comparative inhibitory potencies of BT and HQ correlate with previously published data on their relative facility for autooxidation to quinones at physiological pH. Catechol had no effect at the concentrations employed. Macrophages treated with BQ and BT failed to recover their Fc receptor-mediated phagocytic capacity when incubated overnight in the absence of the xenobiotics. Only small differences in the inhibition of Fc receptor-mediated phagocytosis were observed between macrophages exposed to BQ at 4 versus 37 degrees C. BQ also had little effect on the Fc receptor binding of target cells. Fluorescent digital imaging microscopy demonstrated that BQ treatment markedly decreased the filamentous actin content of macrophages. However, BQ bound in low amounts to purified actin and did not affect its assembly. Our findings suggest that a mechanism for inhibition of Fc receptor-mediated phagocytosis by BQ is disruption of filamentous actin via an effect(s) other than the direct alkylation of actin by BQ.