Analysis of the structural diversity of mycolic acids of Rhodococcus and Gordonla isolates from activated sludge foams by selective ion monitoring gas chromatography–mass spectrometry (SIM GC–MS)

• Published in: Journal of microbiological methods Vol. 35; no. 1; pp. 53 - 63
• Main Authors: Stratton, H.M, Brooks, P.R, Seviour, R.J
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01-02-1999; Elsevier Science
• Subjects: Bacteriology; Biological and medical sciences; Chemotaxonomy; Fundamental and applied biological sciences. Psychology; GC–MS; Gordonla; Microbiology; Morphology, structure, chemical composition; Mycolic acids; Rhodococcus; SIM; Chemotaxonomy; Mycolic acids; Rhodococcus; GC–MS; Gordonla; SIM; Activated sludge; Gordona; GC rich sequence; Variability; Chemical structure; Bacteria; Actinomycetes; Mass spectrometer; Structural analysis
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/S0167-7012(98)00102-X

A method using Selective Ion Monitoring (SIM) gas chromatography–mass spectrometry is described for analysis of mycolic acids which reveals a hitherto unrecognised chemical structural diversity among these in some members of the Mycolata. The structural interpretation of mass spectral data of mycolic acids from
Rhodococcus spp and
Gordonla spp using SIM is discussed.