Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni

• Published in: Carbohydrate polymer technologies and applications Vol. 6; p. 100384
• Main Authors: Sandoval Larios, Giovanna, Huerta Ocampo, José Ángel, Sarabia Sainz, Jose Andrei, García Galaz, Alfonso, Guzmán-Partida, Ana María, Madera Santana, Tomás Jesús, Ramos-Clamont Montfort, Gabriela
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-12-2023; Elsevier
• Subjects: Biorecognition; Campylobacter jejuni; Core-shell nanoparticles; Fucoidan neoglycans; Glycomimetic ligands; Maillard fucosylation; Core-shell nanoparticles; Biorecognition; Maillard fucosylation; Fucoidan neoglycans; Campylobacter jejuni; Glycomimetic ligands
• ISSN: 2666-8939; 2666-8939
• DOI: 10.1016/j.carpta.2023.100384

•Fucoidan oligosaccharides (OFuc) were obtained by mild controlled hydrolysis and separated by ultrafiltration.•The oligosaccharide fraction >1 kDa and <3 kDa was recognized by fucose-specific lectins and subsequently conjugated with bovine serum albumin (BSA-OFuc) by controlled maillard reaction.•Core-shell nanoparticles were successfully functionalized with BSA-OFuc neoglycans and selectively recognized by campylobacter jejuni.
Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core-shell nanoparticles from fucoidan neoglycans as potential glycomimetic ligands for Campylobacter jejuni. Fucoidan oligosaccharides (OFuc) were obtained by mild hydrolysis of fucoidan. OFuc were separated by ultrafiltration, characterized by dynamic light scattering (DLS), infrared spectroscopy (FTIR), Fluorophore-assisted carbohydrate electrophoresis and lectin recognition. The OFuc1 were then conjugated with bovine serum albumin (BSA) by controlled glycation. The formation of fucosylated BSA (BSA-OFuc1) was confirmed by FTIR, increased surface charge, polyacrylamide gel electrophoresis, mass spectrometry and Ulex europaeus I lectin recognition. Afterwards core-shell BSA/BSA-OFuc1 nanoparticles were obtained by crosslinking. These nanoparticles presented hemispherical form with size and charge distribution of 312.1 ± 3.2 nm and -34.4 ± 0.3 mV respectively and were selectively recognized by C. jejuni by an ELISA-like lectin assay (ELLA). These results revel the potential of core-shell nanoparticles from fucoidan neoglycans as glycomimetic ligands for Campylobacter jejuni and open further opportunities to explore potential applications in the pharmaceutic industry.
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