Construction of expression vectors and study on single-chain antibody and reshaping single-domain antibody against CD3

Two vectors, pWA180 and pROH80, for expression of single-chain Fv fragments (ScFv) were con-siruciea. (?)ne anti-CD3 VH and VL genes were amplified from UCHTl cells by RT-PCR and sequenced. Both genes were cloned in pWA180 to express native ScFv and pROH80 for GST-ScFv fusion protein expression. The...

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Bibliographic Details
Published in:	Science China. Life sciences Vol. 40; no. 3; pp. 270 - 276
Main Author:	刘喜富萧飒顾征王勇张卫国陈艾林晴黄华梁孙健陈润生沈倍奋陈兴
Format:	Journal Article
Language:	English
Published:	China Springer Nature B.V 01-06-1997
Subjects:	Antibodies antibody CD3 antigen Construction expression Expression vectors fragment Fusion protein Genes glutathione GST Immunoglobulins Inclusion bodies modeling molecular Nanobodies Oligonucleotides Proteins reshaping s-transferase ScFv single-chain vector
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