Effects of caffeic acid phenethyl ester (CAPE) on angiogenesis, apoptosis and oxidatıve stress ın various cancer cell lines

• Published in: Biotechnic & histochemistry Vol. 94; no. 7; p. 491
• Main Authors: Fırat, F, Özgül, M, Türköz Uluer, E, Inan, S
• Format: Journal Article
• Language: English
• Published: England 03-10-2019
• Subjects: Angiogenesis Inducing Agents - pharmacology; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Caffeic Acids - pharmacology; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Humans; Neovascularization, Pathologic - metabolism; Oxidative Stress - drug effects; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - pharmacology; Propolis - pharmacology; Angiogenesis; apoptosis; cancer; CAPE; oxidative stress; caffeic acid phenethyl ester
• DOI: 10.1080/10520295.2019.1589574
• EISSN: 1473-7760

Cancer is a common cause of death worldwide. Approximately 80% of cancer patients use complementary or alternative medicines for treatment. Caffeic acid phenethyl ester (CAPE), the main active component of propolis, exhibits cytotoxic, antiproliferative and anti-cancer effects. Despite its anticancer effects CAPE exhibits no known harmful effects toward normal cells. We investigated the effects of CAPE on angiogenesis, apoptosis and oxidative stress using MDA MB-231, N2a and COLO 320 cell lines and CAPE treatments at 24 and 48 h. A two dimensional cell culture system was used and the findings were evaluated by an indirect immunohistochemical method and H-scores were calculated. CAPE was effective for all three cancer cell lines. After 24 and 48 h, we found a significant decrease in live cells and increased stress in the cells based on e-NOS and i-NOS levels.