Molecular identification and phylogenetic analysis of Siluriformes from the Paraguay River basin, Brazil

• Published in: Mitochondrial DNA. Part A. DNA mapping, sequencing, and analysis Vol. 28; no. 4; p. 536
• Main Authors: de Carvalho Lima, Thalles Policarpo, do Egito, Andréa Alves, Feijó, Gelson Luís Dias, de Arruda Mauro, Rodiney, Ferraz, André Luiz Julien
• Format: Journal Article
• Language: English
• Published: England 04-07-2017
• Subjects: Animals; Brazil; Catfishes - classification; Catfishes - genetics; DNA Barcoding, Taxonomic - methods; DNA, Mitochondrial - genetics; Fish Proteins - genetics; Phylogeny; Rivers; Pantanal; Catfish; COI; freshwater fish; mitochondrial DNA; neotropics
• ISSN: 2470-1408
• DOI: 10.3109/24701394.2016.1149825

The objective of this study was to identify, through the DNA barcode, fishable Siluriformes which were collected from the Paraguay River basin in Pantanal. It was analyzed for genetic distance calculation using the Kimura-two-model parameters and the dendrogram was builtusing the Neighbour-Joining algorithm. The average genetic distance within species, genus and families were 0.2%, 1.6% and 4.2%, respectively. These values were lower than those reported in studies from other continents, probably due to the recent radiation process undergone by Neotropical fish. The dendrogram revealed two possible cases of hybridization, one individual Pseudoplatystoma corruscans, it was not possible to identify whether it was a natural event or commercial production exhaust and other of Pimelodus cf. argenteus leading to the assumption that the aspects of reproductive isolation cannot be clearly defined. Besides, the populations of the species Hemisorubim platyrhynchos and e Platydora armatulus may be undergoing a substructuring process, with genetic differences 3% and 4%, respectively.