Detection and Whole-Genome Analysis of a High-Risk Clone of Klebsiella pneumoniae ST340/CG258 Producing CTX-M-15 in a Companion Animal

• Published in: Microbial drug resistance (Larchmont, N.Y.) Vol. 26; no. 6; p. 611
• Main Authors: Valencia-Bacca, Juan, Silva, Meire M, Cerdeira, Louise, Esposito, Fernanda, Cardoso, Brenda, Muñoz, Maria E, Jiménez-Villegas, Tatiana, Cardenas-Arias, Adriana, Pessoa, Danielle A N, Lincopan, Nilton
• Format: Journal Article
• Language: English
• Published: United States 01-06-2020
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; beta-Lactamases - genetics; Cats - microbiology; Drug Resistance, Multiple, Bacterial - drug effects; Drug Resistance, Multiple, Bacterial - genetics; Genes, Bacterial; Genome-Wide Association Study; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Male; Microbial Sensitivity Tests; Pets - microbiology; pets; Brazil; ESBL; bacterial pathogens; One Health; multidrug-resistant
• ISSN: 1931-8448
• DOI: 10.1089/mdr.2019.0190

The emergence and dissemination of high-risk clones of
producing extended-spectrum β-lactamases (ESBLs) in animal infections is a critical issue. We report the detection and genomic features of a multidrug-resistant (MDR) ESBL (CTX-M-15)-producing
infecting a domestic cat. Whole-genome sequencing analysis identified the international ST340 (clonal group CG258), and genes and mutations conferring resistance to β-lactams, aminoglycosides, macrolides, phenicols, fosfomycin, sulfonamides, tetracycline, trimethoprim, and fluoroquinolones. In addition, the presence of genes encoding resistance to disinfectant and heavy metals hazardous to humans was also confirmed. The MDR profile exhibited by the strain contributed to treatment failure and death of the companion animal. Therefore, active surveillance of critical priority lineages of
should not only focus on human infections but also on veterinary infections.