Application of CE-ICP-MS and CE-ESI-MS/MS for identification of Zn-binding ligands in Goji berries extracts

• Published in: Talanta (Oxford) Vol. 183; pp. 102 - 107
• Main Authors: Ruzik, Lena, Kwiatkowski, Piotr
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-06-2018
• Subjects: CE-ESI-MS; CE-ICP-MS; Goji berry; Zinc; Goji berry; CE-ICP-MS; Zinc; CE-ESI-MS
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.02.040

The identification of groups of ligands binding metals is a crucial issue for the better understanding of their bioaccessibility. In the current study, we have intended an approach for identification of Zn-binding ligands based on using capillary electrophoresis combined with inductively coupled plasma mass spectrometry (CE-ICP-MS) and tandem electrospray ionization mass spectrometry (CE-ESI-MS/MS). The approach, which featured the use of the coupling of capillary electrophoresis with inductively coupled plasma mass spectrometry allows to separate and observe zinc ions present in complexes with respect to their size and charge and to identify nine compounds with zinc isotopic profile. CE-ICP-MS provides us with information about presence of zinc species and elemental information about zinc distribution. CE-ESI-MS/MS provide us with information about the most favorable Zn binding ligands: amino acids, flavonols, stilbenoids, fenolic acids and carotenoids.
The presented work is the continuation of previous studies based on using LC-ESI-MS/MS, though, now we presented a new solutions with the possibility of changing detectors without changing the separation techniques, what is important without re-optimizing the method. The new presented method allows to identify the zinc-binding ligands in shorter time.
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•Speciation analysis of Zn in goji berries extracts is examined.•Detection and identification of Zn-binding ligands based on using CE-ICP-MS and CE-ESI-MS/MS.•Zn binding ligands: amino acids, flavonols, stilbenoids, fenolic acids and carotenoids were identified by CE-ESI-MS/MS.•Two compatible techniques: CE-ICP-MS for physiological buffer and molecular identification by CE-ESI-MS/MS was examined.