Isotype Determination of Anti-Trypanosoma cruzi Antibody in Murine Chagas' Disease

• Published in: The Journal of parasitology Vol. 78; no. 3; pp. 557 - 561
• Main Authors: Rowland, E. C., Mikhail, K. S., McCormick, T. S.
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01-06-1992
• Subjects: Animals; Antibodies; Antibodies, Protozoan - blood; Antigens; Antigens, Protozoan - immunology; Biological and medical sciences; Blotting, Western; Chagas disease; Chagas Disease - immunology; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Epitopes - immunology; Experimental protozoal diseases and models; Female; Immunization; Immunoglobulin G - blood; Immunoglobulin M - blood; Immunology; Infections; Infectious diseases; Isotypes; Medical sciences; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Parasites; Parasitic diseases; Protozoal diseases; Research Notes; Tropical medicine; Trypanosoma cruzi; Trypanosoma cruzi - immunology; Protozoa; Animal model; Protozoal disease; Antibody; Parasite; Chagas disease; Isotype; Immunological method; Trypanosoma cruzi; Infection; Antigen; Rhizoflagellata; Immunoblotting assay; Trypanosomiasis; ELISA assay; Humoral immunity
• ISSN: 0022-3395; 1937-2345
• DOI: 10.2307/3283671

Isotypic analysis of anti-parasite humoral responses of C57B1/6 and C3H (He) mice surviving acute Trypanosoma cruzi infection showed that both mouse strains demonstrate IgG1, IgG2a, IgG2b, and IgM enzyme-linked immunosorbent assay titers from days 21 to 300 of infection. Using the western blot technique to determine the antigen specificity of the isotypic responses, 100-day infected C3H mice showed strong IgG1, IgG2a, and IgG2b responses to many antigens, whereas C57B1/6 mice showed weak responses to fewer antigens. Isotype western blots showed that reactivity to the T. cruzi antigen of 75-77 kDa is present in the humoral response of day 21-infected mice that will survive and missing in those that will not survive. In general, surviving immunized C3H mice respond with IgG1, IgG2a, and IgG2b reactions to the 75-77-kDa and other antigens, whereas resistant B6 mice concentrate their anti-T. cruzi response in the IgG2b isotype to the 75-77-kDa antigen. Perhaps induction of ineffective antibody responses to nonprotective antigens is beneficial to the parasite and detrimental to the host.