Controllable encapsulation of α-mangostin with quaternized β-cyclodextrin grafted chitosan using high shear mixing

• Published in: International journal of pharmaceutics Vol. 538; no. 1-2; pp. 21 - 29
• Main Authors: Phunpee, Sarunya, Suktham, Kunat, Surassmo, Suvimol, Jarussophon, Suwatchai, Rungnim, Chompoonut, Soottitantawat, Apinan, Puttipipatkhachorn, Satit, Ruktanonchai, Uracha Rungsardthong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-03-2018
• Subjects: Anti-inflammation; Antimicrobial; Chitosan; Effective entrapment; Inclusion complex; α-mangostin; β-CD; Antimicrobial; β-CD; α-mangostin; Effective entrapment; Chitosan; Inclusion complex; Anti-inflammation
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2017.12.016

The CS chain, β-CD and α-mangostin are represented by grey, cyan and pink surfaces, respectively.
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In this study, the inclusion complex formation between α-mangostin and water-soluble quaternized β-CD grafted-chitosan (QCD-g-CS) was investigated. Inclusion complex formation with encapsulation efficiency (%EE) of 5, 15 and 75% can be varied using high speed homogenizer. Tuning %EE plays a role on physicochemical and biological properties of α-mangostin/QCD-g-CS complex. Molecular dynamics simulations indicate that α-mangostin is included within the hydrophobic β-CD cavity and being absorbed on the QCD-g-CS surface, with these results being confirmed by Fourier transform infrared (FTIR) spectroscopy. Probing the release characteristics of the inclusion complex at various %EE (5%, 15% and 75%) in simulated saliva (pH 6.8) demonstrated that α-mangostin release rates were dependent on % EE (order 5% > 15% > 75%). Additionally, higher antimicrobial and anti-inflammation activities were observed for the inclusion complex than those of free α-mangostin due to enhance the solubility of α-mangostin through the inclusion complex with QCD-g-CS.