Utilization of methyltrioctylammonium chloride as new ionic liquid in pretreatment of sugarcane bagasse for production of cellulase by novel thermophilic bacteria

• Published in: Journal of biotechnology Vol. 317; pp. 34 - 38
• Main Authors: Ejaz, Uroosa, Muhammad, Shoaib, Hashmi, Imran Ali, Ali, Firdous Imran, Sohail, Muhammad
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20-06-2020
• Subjects: Alkali; Bacillales - enzymology; Bacillales - genetics; Bacillales - metabolism; Brevibacillus - enzymology; Brevibacillus - genetics; Brevibacillus - metabolism; Cellulase; Cellulase - metabolism; Cellulose - chemistry; Cellulose - metabolism; Fermentation; Ionic liquid; Ionic Liquids - chemistry; Pretreatment; Quaternary Ammonium Compounds - chemistry; Saccharum - chemistry; Saccharum - metabolism; Sugarcane bagasse; Thermophilic bacteria; Thermophilic bacteria; Alkali; Pretreatment; Cellulase; Ionic liquid; Sugarcane bagasse
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2020.04.013

•Thermophilic bacteria were isolated from less explored mesophilic environments.•Ammonium based ionic liquid was used for the pretreatment of sugarcane bagasse.•Pretreated sugarcane bagasse was used for cellulase production from thermophiles.•The promising strains belonged to A. thermoaerophilus and B. borstelensis.
Fermentation of carbohydrates present in lignocellulosic (LC) biomass is facilitated by lignin removal, which is usually achieved by adopting various pretreatment methods to provide the enzymes proper access to their respective substrates. Pretreatment using ionic liquid (IL) is relatively recent advancement and considered as mild and green process. ILs can dissolve extensive quantities of biomass and depolymerize the cellulose. In this context, an abundantly available LC biomass, sugarcane bagasse (SB), was pretreated using alkali or with an IL, methyltrioctylammonium chloride, and was used for cellulase production from thermophilic bacteria. In all, 26 indigenously isolated thermophilic bacterial strains were quantitatively screened for cellulase production. 16S rDNA sequences of the promising isolates UE10 and UE27 revealed relatedness with Brevibacillus borstelensis, while the strain UE1 belonged to Aneurinibacillus thermoaerophilus. Cellulase production was compared by utilizing alkali pretreated and IL pretreated SB and the later was found more appropriate. UE1, UE10 and UE27 yielded 22.2, 22.18 and 33.3 IU mL−1 of endoglucanase, respectively, by fermenting IL pretreated SB. The changes in SB structure after pretreatment were evaluated by scanning electron microscopy. This study demonstrated the potential of novel thermophilic bacterial strains to utilize IL pretreated SB for production of industrially important enzyme, cellulase.