Transcription factors NF-kB, HIF-1, HIF-2, growth factor VEGF, VEGFR2 and carboanhydrase IX mRNA and protein level in the development of kidney cancer metastasis

• Published in: Molecular biology (New York) Vol. 51; no. 2; pp. 328 - 332
• Main Authors: Spirina, L. V., Usynin, Y. A., Yurmazov, Z. A., Slonimskaya, E. M., Kolegova, E. S., Kondakova, I. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-03-2017; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Cancer; Clear cell-type renal cell carcinoma; Enzyme-linked immunosorbent assay; Gene expression; Human Genetics; Hypoxia-inducible factor 1; Hypoxia-inducible factor 1a; Kidney cancer; Life Sciences; Metastases; Metastasis; Molecular Cell Biology; NF-κB protein; Polymerase chain reaction; Proteins; Tissues; Transcription factors; Tumors; Vascular endothelial growth factor; VEGFR2; kidney cancer; HIF-2; carboanhydrase IX; metastasis; VEGF; NF-kB; transcription factors HIF-1α
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893317020194

Here, we have investigated the participation of nuclear factors NF-kB, HIF-1 and HIF-2, VEGF, VEGFR2, and carboanhydrase IX in clear-cell renal cancer. We have determined the expression and protein level of transcription factors, VEGF, VEGFR2, and carboanhydrase IX in tumor and normal tissues of 30 patients with kidney cancer. The Real-Time PCR and ELISA were used in the study. The low levels of HIF-1 mRNA expression associated with high levels of HIF-1 protein were also associated with metastasis. The expression levels of VEGF, VEGFR2, and their protein levels are increased in primary tumors of patients with disseminated kidney cancer compared to nonmetastatic cancer. No correlation was revealed between the content of mRNA and encoded proteins in the kidney cancer tissues. The changes in the ratios of mRNA levels and the respective proteins (HIF-1α, HIF-2, NF-kB, VEGF, VEGFR2, and carboanhydrase IX) may contribute to kidney- cancer metastasis.