Exploring the therapeutic potential of Cynanchum tunicatum (Retz.) Alston- assessment of phytochemicals and biological activities

• Published in: Journal of King Saud University. Science Vol. 36; no. 7; p. 103238
• Main Authors: Krishnamoorthy, Deepika, Swaminathan, Amutha, AlGarawi, Amal Mohamed, Nallasamy, Lavanya, Murugavelu, Girija Sangari, Selvaraj, Swarna Lakshmi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-08-2024; Elsevier
• Subjects: And phytochemical analysis; Anti-inflammatory activity; Antioxidant activity; Cynanchum tunicatum; Anti-inflammatory activity; And phytochemical analysis; Antioxidant activity; Cynanchum tunicatum
• ISSN: 1018-3647
• DOI: 10.1016/j.jksus.2024.103238

Cynanchum tunicatum (Retz.) Alston is native to the Asian region and is distributed in the tropical areas of India and Sri Lanka. The aim of this study is to explore the phytochemical composition, antioxidant potential, and anti-inflammatory properties of C. tunicatum. Metabolic profiling was carried out using phytochemical screening to detect and quantify the secondary metabolites. To evaluate the potential secondary metabolites using the standard methods, Fourier transform infrared (FTIR), High-Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography–Mass Spectrometry (GC–MS) from organic extracts of C. tunicatum and its biological activities. FTIR investigated peaks that represent alkane and aromatic compounds. GC–MS revealed the presence of 22 constituents such as 1-Hexacosene (0.145 %), l-(+)-Ascorbic acid 2,6-dihexadecanoate (8.129 %), Campesterol (5.243 %), Beta −Amyrin (10.614 %), Lupeol (13.061 %), Octadecanoic acid (0.751 %) are the major active compounds present. HPTLC fingerprinting confirms the bioactive compounds such as colchicine, strychnine, coumarin etc. which are represented with corresponding Rf values. Among all the extracts of C. tunicatum methanolic extract showed highest antioxidant activities. In 2,2-diphenylpicrylhydrazyl method exhibit the IC50 of (38.91 µg/mL), Ferric reducing antioxidant power assay (1.6 µg/mL), total antioxidant assay (IC50 = 32.91 µg/mL) and IC50 of anti-inflammatory activity (42.31 µg/mL) respectively. These findings enrich the knowledge of the species Cynanchum tunicatum for the possible application as a source of bioactive compounds in drug discovery.