Prototype isochoric preservation device for large organs
This paper presents the design and prototype of a constant volume (isochoric) vessel that can be used for the preservation of large organs in a supercooled state. This prototype is a preliminary version of a more advanced design. The device consists of a cooling bath operated by a mechanical vapor c...
Saved in:
| Published in: | Frontiers in bioengineering and biotechnology Vol. 12; p. 1335638 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Switzerland
Frontiers Media S.A
2024
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | This paper presents the design and prototype of a constant volume (isochoric) vessel that can be used for the preservation of large organs in a supercooled state. This prototype is a preliminary version of a more advanced design. The device consists of a cooling bath operated by a mechanical vapor compression refrigeration unit and an isochoric chamber made of stainless steel. The preservation of organs using supercooling technology in an isochoric chamber requires a continuous temperature and pressure monitoring. While the device was initially designed for pig liver experiments, its innovative design and preservation capabilities suggest potential applications for preserving other organs as well. The isochoric reactor may be used to accommodate a variety of organ types, opening the door for further research into its multi-organ preservation capabilities. All the design details are presented in this study with the purpose of encouraging researchers in the field to build their own devices, and by this to improve the design. We chose to design the device for isochoric supercooling as the method of preservation to avoid the ice formation. |
|---|---|
| AbstractList | This paper presents the design and prototype of a constant volume (isochoric) vessel that can be used for the preservation of large organs in a supercooled state. This prototype is a preliminary version of a more advanced design. The device consists of a cooling bath operated by a mechanical vapor compression refrigeration unit and an isochoric chamber made of stainless steel. The preservation of organs using supercooling technology in an isochoric chamber requires a continuous temperature and pressure monitoring. While the device was initially designed for pig liver experiments, its innovative design and preservation capabilities suggest potential applications for preserving other organs as well. The isochoric reactor may be used to accommodate a variety of organ types, opening the door for further research into its multi-organ preservation capabilities. All the design details are presented in this study with the purpose of encouraging researchers in the field to build their own devices, and by this to improve the design. We chose to design the device for isochoric supercooling as the method of preservation to avoid the ice formation. |
| Author | Neacșu, Ion Năstase, Gabriel Șerban, Alexandru Popescu, Irinel Beșchea, George-Andrei Fetecău, Cătălin Botea, Florin Câmpean, Ștefan-Ioan |
| Author_xml | – sequence: 1 givenname: Alexandru surname: Șerban fullname: Șerban, Alexandru organization: Politechnica University of Bucharest, Bucharest, Romania – sequence: 2 givenname: Gabriel surname: Năstase fullname: Năstase, Gabriel organization: Department of Building Services, Transilvania University of Brasov, Brasov, Romania – sequence: 3 givenname: George-Andrei surname: Beșchea fullname: Beșchea, George-Andrei organization: Department of Building Services, Transilvania University of Brasov, Brasov, Romania – sequence: 4 givenname: Ștefan-Ioan surname: Câmpean fullname: Câmpean, Ștefan-Ioan organization: Department of Building Services, Transilvania University of Brasov, Brasov, Romania – sequence: 5 givenname: Cătălin surname: Fetecău fullname: Fetecău, Cătălin organization: Faculty of Mechanical Engineering, Dunarea de Jos University of Galati, Galati, Romania – sequence: 6 givenname: Irinel surname: Popescu fullname: Popescu, Irinel organization: Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania – sequence: 7 givenname: Florin surname: Botea fullname: Botea, Florin organization: Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, Bucharest, Romania – sequence: 8 givenname: Ion surname: Neacșu fullname: Neacșu, Ion organization: Criomec SA, Galati, Romania |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38524196$$D View this record in MEDLINE/PubMed |
| BookMark | eNpNUU1LAzEQDaL4_Qc8yB69bM33JkcpfhQKetBzyGYnGtluarIt-O-NbS0yhxmG99485p2hwyEOgNAVwRPGlL71bYgwoZjyCWFMSKYO0CmlWtacKHH4bz5Blzl_YowJFY1Q9BidMCUoJ1qeIvWS4hjH7yVUIUf3EVNw1TJBhrS2Y4hD1cE6OKh8TFVv0ztUMb3bIV-gI2_7DJe7fo7eHu5fp0_1_PlxNr2b145JPtZad1gB5YK4TjknHW-p9523HXEeOm2BsUZzaZsCxGAbLlVjMZOYtaoVjJ2j2Va3i_bTLFNY2PRtog1msyhmjE1jcD0YTa1WngjgmnKqVSlQpDiQwnqmoWjdbLWWKX6tII9mEbKDvrcDxFU2hSMaLTDRBUq3UJdizgn8_jTB5jcAswnA_AZgdgEU0vVOf9UuoNtT_t7NfgANj4Mb |
| Cites_doi | 10.3390/bioengineering10080934 10.1038/s42005-020-0303-9 10.1111/ijfs.14463 10.1006/cryo.1999.2205 10.1016/j.ifset.2018.10.016 10.1016/j.postharvbio.2022.112072 10.1371/journal.pone.0183353 10.1063/5.0051662 10.1016/j.athoracsur.2003.04.004 10.1038/nprot.2015.011 10.1016/J.BBRC.2005.09.082 10.1016/j.cryobiol.2006.04.001 10.1103/PhysRevResearch.2.023350 10.1063/1.5145334 10.1016/j.bbrc.2021.04.084 10.1038/s41467-018-05636-0 10.1016/j.cryobiol.2018.06.005 10.1038/s41587-019-0223-y 10.1016/j.cryobiol.2015.04.010 10.1016/j.cryobiol.2004.12.002 10.1016/j.cryobiol.2009.06.010 10.1038/s41467-022-31490-2 10.1016/j.foodres.2021.110228 10.1038/s42003-021-02650-9 10.1038/nm.3588 10.1115/1.4043521 10.1016/j.cryobiol.2022.03.003 10.1006/cryo.1999.2225 |
| ContentType | Journal Article |
| Copyright | Copyright © 2024 Șerban, Năstase, Beșchea, Câmpean, Fetecău, Popescu, Botea and Neacșu. |
| Copyright_xml | – notice: Copyright © 2024 Șerban, Năstase, Beșchea, Câmpean, Fetecău, Popescu, Botea and Neacșu. |
| DBID | NPM AAYXX CITATION 7X8 DOA |
| DOI | 10.3389/fbioe.2024.1335638 |
| DatabaseName | PubMed CrossRef MEDLINE - Academic Directory of Open Access Journals |
| DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
| DatabaseTitleList | CrossRef MEDLINE - Academic PubMed |
| Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering |
| EISSN | 2296-4185 |
| EndPage | 1335638 |
| ExternalDocumentID | oai_doaj_org_article_92a98f15e4924298989e8199d65af39e 10_3389_fbioe_2024_1335638 38524196 |
| Genre | Journal Article |
| GroupedDBID | 53G 5VS 9T4 AAFWJ ACGFS ACXDI ADBBV ADRAZ ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV DIK GROUPED_DOAJ GX1 HYE IAO IEA IHR IPNFZ ISR KQ8 M48 M~E NPM OK1 PGMZT RIG RPM AAYXX CITATION 7X8 |
| ID | FETCH-LOGICAL-c364t-99d08e2451cd8cc6c4b2ffdfad1cfed9ae337946a79d00ea74687a03603b8b533 |
| IEDL.DBID | DOA |
| ISSN | 2296-4185 |
| IngestDate | Tue Oct 22 15:15:07 EDT 2024 Sat Oct 26 05:59:05 EDT 2024 Thu Nov 21 23:44:04 EST 2024 Sat Nov 02 12:05:10 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Keywords | prototype development subfreezing temperatures cryopreservation isochoric organ preservation |
| Language | English |
| License | Copyright © 2024 Șerban, Năstase, Beșchea, Câmpean, Fetecău, Popescu, Botea and Neacșu. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c364t-99d08e2451cd8cc6c4b2ffdfad1cfed9ae337946a79d00ea74687a03603b8b533 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://doaj.org/article/92a98f15e4924298989e8199d65af39e |
| PMID | 38524196 |
| PQID | 2985795019 |
| PQPubID | 23479 |
| PageCount | 1 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_92a98f15e4924298989e8199d65af39e proquest_miscellaneous_2985795019 crossref_primary_10_3389_fbioe_2024_1335638 pubmed_primary_38524196 |
| PublicationCentury | 2000 |
| PublicationDate | 2024-00-00 |
| PublicationDateYYYYMMDD | 2024-01-01 |
| PublicationDate_xml | – year: 2024 text: 2024-00-00 |
| PublicationDecade | 2020 |
| PublicationPlace | Switzerland |
| PublicationPlace_xml | – name: Switzerland |
| PublicationTitle | Frontiers in bioengineering and biotechnology |
| PublicationTitleAlternate | Front Bioeng Biotechnol |
| PublicationYear | 2024 |
| Publisher | Frontiers Media S.A |
| Publisher_xml | – name: Frontiers Media S.A |
| References | Zhang (B34) 2018; 83 Rubinsky (B28) 2022 Bilbao-sainz (B4) 2022; 194 Puts (B27) 2015; 71 Szobota (B31) 2006; 53 Ishine (B15) 2000; 40 Berendsen (B2) 2014; 20 Ishine (B14) 1999; 39 Huang (B13) 2018; 9 Consiglio (B10) 2020; 2 Powell-Palm (B23) 2022 Rubinsky (B29) 2020 Powell-Palm (B20) 2019; 141 Powell-Palm (B21) 2021; 4 Bilbao-Sainz (B6) 2020; 55 Bilbao-Sainz (B5) 2019; 52 Preciado (B25) 2007 de Vries (B12) 2019; 37 Ukpai (B33) 2017; 12 Tessier (B32) 2022; 13 Amir (B1) 2004; 77 Perez (B19) 2007 Powell-Palm (B22); 116 Bilbao-Sainz (B7) 2021; 143 Preciado (B26) 2010; 60 Câmpean (B9) 2021; 11 Powell-Palm (B24); 3 Bruinsma (B8) 2015; 10 Luyet (B16) 1952 Beschea (B3) 2021; 559 Consiglio (B11) 2022; 106 Monzen (B17) 2005; 337 Rubinsky (B30) 2005; 50 Năstase (B18) 2023; 10 |
| References_xml | – volume: 10 start-page: 934 year: 2023 ident: B18 article-title: Isochoric supercooling organ preservation system publication-title: Bioengineering doi: 10.3390/bioengineering10080934 contributor: fullname: Năstase – volume: 3 start-page: 39 ident: B24 article-title: Freezing water at constant volume and under confinement publication-title: Commun. Phys. doi: 10.1038/s42005-020-0303-9 contributor: fullname: Powell-Palm – volume: 55 start-page: 2141 year: 2020 ident: B6 article-title: Preservation of spinach by isochoric (constant volume) freezing publication-title: Int. J. Food Sci. Technol. doi: 10.1111/ijfs.14463 contributor: fullname: Bilbao-Sainz – volume: 39 start-page: 271 year: 1999 ident: B14 article-title: A histological analysis of liver injury in freezing storage publication-title: Cryobiology doi: 10.1006/cryo.1999.2205 contributor: fullname: Ishine – start-page: 63351825 volume-title: Device and method to reduce the probability of ice nucleation during preservation of biological matter in isochoric (constant volume) systems year: 2022 ident: B28 contributor: fullname: Rubinsky – volume: 52 start-page: 108 year: 2019 ident: B5 article-title: Preservation of sweet cherry by isochoric (constant volume) freezing publication-title: Innovative Food Sci. Emerg. Technol. doi: 10.1016/j.ifset.2018.10.016 contributor: fullname: Bilbao-Sainz – volume-title: Thermodynamics and heat transfer analysis for isochoric cryopreservation year: 2007 ident: B19 contributor: fullname: Perez – volume: 194 start-page: 112072 year: 2022 ident: B4 article-title: Isochoric freezing and isochoric supercooling as innovative postharvest technologies for pomegranate preservation publication-title: Postharvest Biol. Technol. doi: 10.1016/j.postharvbio.2022.112072 contributor: fullname: Bilbao-sainz – volume: 12 start-page: e0183353 year: 2017 ident: B33 article-title: Pressure in isochoric systems containing aqueous solutions at subzero centigrade temperatures publication-title: PLoS One doi: 10.1371/journal.pone.0183353 contributor: fullname: Ukpai – volume: 11 start-page: 11 year: 2021 ident: B9 article-title: Analysis of the relative supercooling enhancement of two emerging supercooling techniques publication-title: AIP Adv. doi: 10.1063/5.0051662 contributor: fullname: Câmpean – volume: 77 start-page: 1648 year: 2004 ident: B1 article-title: Pro-longed 24-hour subzero preservation of heterotopically transplanted rat hearts using antifreeze proteins de-rived from arctic fish publication-title: Ann. Thorac. Surg. doi: 10.1016/j.athoracsur.2003.04.004 contributor: fullname: Amir – volume-title: Devices and methods for high-stability supercooling of aqueous media and bio-logical matter year: 2022 ident: B23 contributor: fullname: Powell-Palm – volume-title: The fundamentals of isochoric freezing and its role in the cryopreservation of biological materials year: 2007 ident: B25 contributor: fullname: Preciado – volume: 10 start-page: 484 year: 2015 ident: B8 article-title: Supercooling preservation and transplantation of the rat liver publication-title: Nat. Protoc. doi: 10.1038/nprot.2015.011 contributor: fullname: Bruinsma – start-page: 746 year: 1952 ident: B16 article-title: On the supercooling of water publication-title: Phys. Rev. contributor: fullname: Luyet – volume: 337 start-page: 534 year: 2005 ident: B17 article-title: The use of a supercooling refrigerator improves the preservation of organ grafts publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/J.BBRC.2005.09.082 contributor: fullname: Monzen – volume: 53 start-page: 139 year: 2006 ident: B31 article-title: Analysis of isochoric subcooling publication-title: Cryobiology doi: 10.1016/j.cryobiol.2006.04.001 contributor: fullname: Szobota – volume: 2 start-page: 023350 year: 2020 ident: B10 article-title: Suppression of cavitation-induced nucleation in sys-tems under isochoric confinement publication-title: Phys. Rev. Res. doi: 10.1103/PhysRevResearch.2.023350 contributor: fullname: Consiglio – volume: 116 ident: B22 article-title: Isochoric conditions enhance stability of metastable supercooled water publication-title: Appl. Phys. Lett. doi: 10.1063/1.5145334 contributor: fullname: Powell-Palm – volume: 559 start-page: 42 year: 2021 ident: B3 article-title: Glucose and glycerol temperature-pressure correlations for the design of cryopreservation protocols in an isochoric system at sub-freezing temperature publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2021.04.084 contributor: fullname: Beschea – volume: 9 start-page: 3201 year: 2018 ident: B13 article-title: Long-term deep-supercooling of large-volume water and red cell sus-pensions via surface sealing with immiscible liquids publication-title: Nat. Commun. doi: 10.1038/s41467-018-05636-0 contributor: fullname: Huang – volume: 83 start-page: 48 year: 2018 ident: B34 article-title: Isochoric vitrification: an experimental study to establish proof of concept publication-title: Cryobiology doi: 10.1016/j.cryobiol.2018.06.005 contributor: fullname: Zhang – volume: 37 start-page: 1131 year: 2019 ident: B12 article-title: Supercooling extends preservation time of human livers publication-title: Nat. Biotechnol. doi: 10.1038/s41587-019-0223-y contributor: fullname: de Vries – volume: 71 start-page: 125 year: 2015 ident: B27 article-title: Polyethylene glycol protects primary hepatocytes during supercooling preservation publication-title: Cryobiology doi: 10.1016/j.cryobiol.2015.04.010 contributor: fullname: Puts – volume: 50 start-page: 121 year: 2005 ident: B30 article-title: The thermodynamic principles of isochoric cryopreservation publication-title: Cryobiol-ogy doi: 10.1016/j.cryobiol.2004.12.002 contributor: fullname: Rubinsky – volume-title: Process and devices for temperature and pressure controlled cryopreserva-tion year: 2020 ident: B29 contributor: fullname: Rubinsky – volume: 60 start-page: 23 year: 2010 ident: B26 article-title: Isochoric preservation: a novel characterization method publication-title: Cryobiology doi: 10.1016/j.cryobiol.2009.06.010 contributor: fullname: Preciado – volume: 13 start-page: 4008 year: 2022 ident: B32 article-title: Partial freezing of rat livers extends preservation time by 5-fold publication-title: Nat. Commun. doi: 10.1038/s41467-022-31490-2 contributor: fullname: Tessier – volume: 143 start-page: 110228 year: 2021 ident: B7 article-title: Preservation of grape tomato by isochoric freezing publication-title: Food Res. Int. doi: 10.1016/j.foodres.2021.110228 contributor: fullname: Bilbao-Sainz – volume: 4 start-page: 1118 year: 2021 ident: B21 article-title: Isochoric supercooled preser-vation and revival of human cardiac microtissues publication-title: Commun. Biol. doi: 10.1038/s42003-021-02650-9 contributor: fullname: Powell-Palm – volume: 20 start-page: 790 year: 2014 ident: B2 article-title: Supercooling enables long-term transplantation survival following 4 Days of liver preserva-tion publication-title: Nat. Med. doi: 10.1038/nm.3588 contributor: fullname: Berendsen – volume: 141 start-page: 081011 year: 2019 ident: B20 article-title: Thermodynamic theory and experimental validation of a multiphase isochoric freezing process publication-title: J. Biomech. Eng. doi: 10.1115/1.4043521 contributor: fullname: Powell-Palm – volume: 106 start-page: 91 year: 2022 ident: B11 article-title: Methods to stabilize aqueous supercool-ing identified by use of an isochoric nucleation detection (INDe) device publication-title: Cryobiology doi: 10.1016/j.cryobiol.2022.03.003 contributor: fullname: Consiglio – volume: 40 start-page: 84 year: 2000 ident: B15 article-title: Transplantation of mammalian livers following freezing: vascular damage and functional recovery publication-title: Cryobiology doi: 10.1006/cryo.1999.2225 contributor: fullname: Ishine |
| SSID | ssj0001257582 |
| Score | 2.306258 |
| Snippet | This paper presents the design and prototype of a constant volume (isochoric) vessel that can be used for the preservation of large organs in a supercooled... |
| SourceID | doaj proquest crossref pubmed |
| SourceType | Open Website Aggregation Database Index Database |
| StartPage | 1335638 |
| SubjectTerms | cryopreservation isochoric organ preservation prototype development subfreezing temperatures |
| Title | Prototype isochoric preservation device for large organs |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/38524196 https://search.proquest.com/docview/2985795019 https://doaj.org/article/92a98f15e4924298989e8199d65af39e |
| Volume | 12 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA66Jz2Ib-tjqeBN6vaRpMnRxy57EkEFbyFpJ7ggW9nH_3cm7S57ES9eS6DJNyHzzWTyDWM3ULgMgMsEZOoSuihKtM-oytw6DYUsLFBqYPxaPn-opyHJ5KxbfVFNWCsP3AI30LnVymcCOEYKeeh2COjFdC2F9YWGcPqmciOYarMrSENU3r6SwShMD7ybNCSLmfM7DMuEpAcpG54oCPb_zjKDtxnts72OJsb37fQO2BZMD9nuhnjgEVMvs2bRUAo1niDEnyT2EVNZ6yrNGtdAx0CMtDT-ooLvOLRwmh-z99Hw7XGcdH0QkqqQfJHgYlMFORcZPeSvZMVd7n3tbZ1VHmqNcBakE29LHJiCLblUpUXXlBZOOeRzJ6w3baZwxmLOq1I4AdrzEgMzZxW3deXRjFUILSJ2u8LEfLdyFwbDBELQBAQNIWg6BCP2QLCtR5JUdfiAyzGdAc1fBozY9Qp0g1ub7ivsFJrl3OBgUWqBJDRip6011r8qlEDuoeX5f0zhgu3QstrMyiXrLWZLuGLb83rZD9uqH5JAPyDg0L8 |
| link.rule.ids | 315,782,786,866,2106,4028,27932,27933,27934 |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Prototype+isochoric+preservation+device+for+large+organs&rft.jtitle=Frontiers+in+bioengineering+and+biotechnology&rft.au=%C8%98erban%2C+Alexandru&rft.au=N%C4%83stase%2C+Gabriel&rft.au=Be%C8%99chea%2C+George-Andrei&rft.au=C%C3%A2mpean%2C+%C8%98tefan-Ioan&rft.date=2024&rft.issn=2296-4185&rft.eissn=2296-4185&rft.volume=12&rft.spage=1335638&rft.epage=1335638&rft_id=info:doi/10.3389%2Ffbioe.2024.1335638&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2296-4185&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2296-4185&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2296-4185&client=summon |