Histamine-stimulated cytokine secretion from human conjunctival epithelial cells: inhibition by the histamine H1 antagonist emedastine

The present studies demonstrate that histamine induces the secretion of IL-6, IL-8 and GM-CSF from human conjunctival epithelial cells in a dose- and time-dependent manner. The histamine antagonists emedastine (H1), ranitidine (H2) and thioperamide (H3) were evaluated for their ability to inhibit se...

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Bibliographic Details
Published in:	International archives of allergy and immunology Vol. 115; no. 4; p. 288
Main Authors:	Weimer, L K, Gamache, D A, Yanni, J M
Format:	Journal Article
Language:	English
Published:	Switzerland 01-04-1998
Subjects:	Benzimidazoles - pharmacology Cells, Cultured Conjunctiva - drug effects Conjunctiva - secretion Cytokines - secretion Epithelial Cells - secretion Granulocyte-Macrophage Colony-Stimulating Factor - secretion Histamine - pharmacology Histamine H1 Antagonists - pharmacology Humans Interleukin-6 - secretion Interleukin-8 - secretion
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Summary:	The present studies demonstrate that histamine induces the secretion of IL-6, IL-8 and GM-CSF from human conjunctival epithelial cells in a dose- and time-dependent manner. The histamine antagonists emedastine (H1), ranitidine (H2) and thioperamide (H3) were evaluated for their ability to inhibit secretion of these cytokines. Emedastine potently inhibited histamine-induced IL-6, IL-8 and GM-CSF secretion with mean IC50 values of 2.23, 3.42 and 1.50 nM, respectively. Ranitidine and thioperamide failed to inhibit cytokine secretion over a wide dose range. These data suggest that mast cell derived histamine may stimulate inflammatory cytokine production in allergic conjunctivitis via activation of epithelial cell H1 receptors. The histamine H1 antagonist emedastine potently inhibits this response.
ISSN:	1018-2438
DOI:	10.1159/000069459