Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes

Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes

Industrial biocatalysis plays an important role in the development of a sustainable economy, as enzymes can be used to synthesize an enormous range of complex molecules under environmentally friendly conditions. To further develop the field, intensive research is being conducted on process technolog...

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Published in:Advanced materials (Weinheim) Vol. 35; no. 39; p. e2303952
Main Authors: Hertel, Julian S, Bitterwolf, Patrick, Kröll, Sandra, Winterhalter, Astrid, Weber, Annika J, Grösche, Maximilian, Walkowsky, Laurenz B, Heißler, Stefan, Schwotzer, Matthias, Wöll, Christof, van de Kamp, Thomas, Zuber, Marcus, Baumbach, Tilo, Rabe, Kersten S, Niemeyer, Christof M
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01-09-2023
Subjects:
Alcohols
Conjugation
Continuous flow
Enzymes
Foams
Materials science
Microreactors
Self-assembly
Stereoselectivity
Water drops
flow biocatalysis
enzymes
porous materials
microreactors
monodisperse foams
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Summary:Industrial biocatalysis plays an important role in the development of a sustainable economy, as enzymes can be used to synthesize an enormous range of complex molecules under environmentally friendly conditions. To further develop the field, intensive research is being conducted on process technologies for continuous flow biocatalysis in order to immobilize large quantities of enzyme biocatalysts in microstructured flow reactors under conditions that are as gentle as possible in order to realize efficient material conversions. Here, monodisperse foams consisting almost entirely of enzymes covalently linked via SpyCatcher/SpyTag conjugation are reported. The biocatalytic foams are readily available from recombinant enzymes via microfluidic air-in-water droplet formation, can be directly integrated into microreactors, and can be used for biocatalytic conversions after drying. Reactors prepared by this method show surprisingly high stability and biocatalytic activity. The physicochemical characterization of the new materials is described and exemplary applications in biocatalysis are shown using two-enzyme cascades for the stereoselective synthesis of chiral alcohols and the rare sugar tagatose.
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ISSN:0935-9648
1521-4095
DOI:10.1002/adma.202303952