Compensatory renal growth after unilateral or subtotal nephrectomy in the ovine fetus

• Published in: Pediatric research Vol. 74; no. 6; pp. 624 - 632
• Main Authors: Sammut, Sébastien, Behr, Luc, Hekmati, Mehrak, Gubler, Marie Claire, Laborde, Kathleen, Lelièvre Pégorier, Martine
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01-12-2013
• Subjects: 631/136/2060; 692/698/272/1684; 692/700/565/545; Animals; Apoptosis; basic-science-investigation; Cell Proliferation; Kidney - anatomy & histology; Kidney - embryology; Kidney - surgery; Medicine; Medicine & Public Health; Models, Biological; Nephrectomy; Organ Size; Pediatric Surgery; Pediatrics; Sheep - embryology
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/pr.2013.149

Background:
Clinical and experimental studies show that unilateral (1/2Nx) and subtotal nephrectomy (5/6Nx) in adults result in compensatory renal growth without formation of new nephrons. During nephrogenesis, the response to renal mass reduction has not been fully investigated.
Methods:
Ovine fetuses underwent 1/2Nx, 5/6Nx, or sham surgery (sham) at 70 d of gestation (term: 150 d), when nephrogenesis is active. At 134 d, renal function was determined, fetuses were killed, and kidneys were further analyzed at the cellular and molecular levels. Additional fetuses subjected to 5/6Nx were killed at 80 and 90 d of gestation to investigate the kinetics of the renal compensatory process.
Results:
At 134 d, in 1/2Nx, a significant increase in kidney weight and estimated glomerular number was observed. In 5/6Nx, the early and marked catch-up in kidney weight and estimated glomerular number was associated with a striking butterfly-like remodeling of the kidney that developed within the first 10 d following nephrectomy. In all groups,
in utero
glomerular filtration rates were similar.
Conclusion:
Compensatory renal growth was observed after parenchymal reduction in both models; however, the resulting compensatory growth was strikingly different. After 5/6Nx, the remnant kidney displayed a butterfly-like remodeling, and glomerular number was restored.