Electrodes modified with bacteriophages and carbon nanofibres for cysteine detection

• Published in: Sensors and actuators. B, Chemical Vol. 287; pp. 78 - 85
• Main Authors: Szot-Karpińska, Katarzyna, Leśniewski, Adam, Jönsson-Niedziółka, Martin, Marken, Frank, Niedziółka-Jönsson, Joanna
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15-05-2019; Elsevier Science Ltd
• Subjects: Bacteriophage film electrode; Biomarkers; Carbon; Carbon fibers; Carbon nanofibers; Cysteine; Electrocatalysis; Electrodes; l-Cysteine detection; Nanofibers; Oxidation; Phages; Self-assembly; Surface area; Voltammetry; Zeta potential; l-Cysteine detection; Electrocatalysis; Carbon nanofibers; Voltammetry; Bacteriophage film electrode
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2019.01.148

A wild M13 filamentous bacteriophage particles were used for carbon nanofibres (CNF) electrode modification. An application of M13 particles for CNF-based electrode modification leads to better develop electroactive surface, higher capacitive currents, and was successfully applied for the electrocatalytic oxidation of the l-cysteine with catechol mediator.
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•A wild M13 bacteriophage particles were used for carbon nanofibres (CNF) electrode modification.•The use of M13 for CNF electrode modification improves electroactive surface and capacitance current.•The development of electroactive surface of the M13-CNF electrode was studied.•The M13-CNF electrode was successfully applied for the electrocatalytic oxidation of the L-cysteine.•A wild M13 bacteriophage particles are promising building blocks for electrode modification.
Here we exploit the high surface area and ability of wild bacteriophage particles to form self-assembled structures. Utilization of these particles for modifying electrodes with carbon nanofibres (CNF) leads to the generation of a novel hybrid material with highly developed surface and thus an electrode with an extended active area, which is desirable when preparing new sensing platforms. This report helps filling gaps in the current knowledge about bacteriophage-nano-material interactions. The wild bacteriophage-carbon nanofibre interactions were thoroughly characterized by microscopy techniques and zeta potential measurements. Our results show that the electroactive surface area is better developed when the bacteriophages are added to the CNF-based electrodes, compared to the case of only the bare electrode, or an electrode modified only with CNFs. The electrocatalytic response towards the oxidation of the thiol biomarker l-cysteine was evaluated with cyclic voltammetry. The results show that the response of the electrode is improved if bacteriophages are used for CNF-based electrode modification.