Mycotoxin occurrence in breast milk and exposure estimation of lactating mothers using urinary biomarkers in São Paulo, Brazil

• Published in: Environmental pollution (1987) Vol. 279; p. 116938
• Main Authors: Coppa, Carolina F.S.C., Cirelli, Amanda C., Gonçalves, Bruna L., Barnabé, Eliana M.B., Petta, Tânia, Franco, Larissa T., Javanmardi, Fardin, Khaneghah, Amin Mousavi, Lee, Sarah H.I., Corassin, Carlos H., Oliveira, Carlos A.F.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15-06-2021
• Subjects: Biomarkers - urine; Brazil; Exposure assessment; Female; Food Contamination - analysis; Human biomonitoring; Human milk; Humans; Lactation; Milk, Human - chemistry; Mothers; Mycotoxins; Urine; Brazil; Urine; Human milk; Human biomonitoring; Exposure assessment
• ISSN: 0269-7491; 1873-6424
• DOI: 10.1016/j.envpol.2021.116938

In this study, the occurrence of aflatoxins (AFs), fumonisins (FBs), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN) and some of their metabolites were assessed in breast milk and urine of lactating women (N = 74) from Pirassununga, São Paulo, Brazil. Exposure estimations through urinary mycotoxin biomarkers was also performed. Samples were collected in four sampling times (May and August 2018, February and July 2019) and analyzed by liquid chromatography coupled to tandem mass spectrometry. Aflatoxin M1 (AFM1) was not detected in breast milk. However, two samples (3%) presented FB1 at 2200 and 3400 ng/L, while 4 samples (5%) had OTA at the median level of 360 ng/L. In urine, AFM1 and aflatoxin P1 (AFP1) were found in 51 and 11% of samples, respectively (median levels: 0.16 and 0.07 ng/mg creatinine, respectively). Urinary DON (median level: 38.59 ng/mg creatinine), OTA (median level: 2.38 ng/mg creatinine) and ZEN (median level: 0.02 ng/mg of creatinine) were quantified in 18, 8 and 10% of the samples, respectively. Mean probable daily intake (PDI) values based on urinary biomarkers were 1.58, 1.09, 5.07, and 0.05 μg/kg body weight/day for AFM1, DON, OTA, and ZEN, respectively. Although a low mycotoxin occurrence was detected in breast milk, the PDI for the genotoxic AFs was much higher than those reported previously in Brazil, while PDI values obtained for OTA and DON were higher than recommended tolerable daily intakes. These outcomes warrant concern on the exposure of lactating women to these mycotoxins in the studied area.
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•Low frequencies and levels of OTA and FB1 were found in breast milk in São Paulo, Brazil.•AFM1, OTA, DON and ZEN were detected in 62, 8,18, 10% of urine samples, respectively.•PDI based on urinary biomarkers varied from 0.05 to 5.07 μg/kg body weight/day.•Urinary data indicated high exposure of lactating women to dietary AFs, OTA and DON.