Lignocellulolytic characterization and comparative secretome analysis of a Trichoderma erinaceum strain isolated from decaying sugarcane straw

• Published in: Fungal biology Vol. 123; no. 4; pp. 330 - 340
• Main Authors: da Silva, Desireé S., Dantzger, Miriam, Assis, Michelle A., Gallardo, Jéssica C.M., Teixeira, Gleidson S., Missawa, Sílvia K., Domingues, Romênia R., Carazzolle, Marcelo F., Lunardi, Inês, Leme, Adriana F.P., Pereira, Gonçalo A.G., Parreiras, Lucas S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-04-2019
• Subjects: Biofuels; Biotransformation; Fungal Proteins - analysis; Fungal proteomics; Hydrolases - analysis; Hydrolysis; Lignin - metabolism; Lignocellulases; Lignocellulosic biomass; Plant Stems - microbiology; Proteome - analysis; Saccharum - microbiology; Saprotrophic fungi; Trichoderma - chemistry; Trichoderma - isolation & purification; Trichoderma - metabolism; Lignocellulases; Biofuels; Saprotrophic fungi; Fungal proteomics; Lignocellulosic biomass
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2019.01.007

The fungus Trichoderma reesei is employed in the production of most enzyme cocktails used by the lignocellulosic biofuels industry today. Despite significant improvements, the cost of the required enzyme preparations remains high, representing a major obstacle for the industrial production of these alternative fuels. In this study, a new Trichoderma erinaceum strain was isolated from decaying sugarcane straw. The enzyme cocktail secreted by the new isolate during growth in pretreated sugarcane straw-containing medium presented higher specific activities of β-glucosidase, endoxylanase, β-xylosidase and α-galactosidase than the cocktail of a wild T. reesei strain and yielded more glucose in the hydrolysis of pretreated sugarcane straw. A proteomic analysis of the two strains' secretomes identified a total of 86 proteins, of which 48 were exclusive to T. erinaceum, 35 were exclusive to T. reesei and only 3 were common to both strains. The secretome of T. erinaceum also displayed a higher number of carbohydrate-active enzymes than that of T. reesei (37 and 27 enzymes, respectively). Altogether, these results reveal the significant potential of the T. erinaceum species for the production of lignocellulases, both as a possible source of enzymes for the supplementation of industrial cocktails and as a candidate chassis for enzyme production.
•A new Trichoderma erinaceum strain was isolated from decaying sugarcane straw.•The new strain exhibited higher lignocellulolytic enzyme activities than T. reesei.•Hydrolysis of pretreated straw with T. erinaceum's enzymes yielded more glucose.•The new strain's secretome also had a higher number of CAZymes than T. reesei's.