Age-associated development of inflammation in Wistar rats: Effects of caloric restriction

• Published in: Archives of physiology and biochemistry Vol. 117; no. 3; pp. 140 - 150
• Main Authors: Horrillo, Daniel, Sierra, Johanna, Arribas, Carmen, García-San Frutos, Miriam, Carrascosa, José M., Lauzurica, Nuria, Fernández-Agulló, Teresa, Ros, Manuel
• Format: Journal Article
• Language: English
• Published: Colchester Informa Healthcare 01-07-2011; Taylor & Francis; Informa
• Subjects: Adipose Tissue - metabolism; Adipose Tissue - pathology; Ageing; Aging - physiology; Animals; Biological and medical sciences; Biomarkers - metabolism; Caloric Restriction; citokynes; Cytokines - metabolism; Endoplasmic Reticulum - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Hypothalamus - metabolism; Hypothalamus - pathology; infiltration; Inflammation - metabolism; insulin resistance; Insulin Resistance - physiology; Liver - metabolism; Liver - pathology; Macrophages - metabolism; Male; Muscle, Skeletal - metabolism; Muscle, Skeletal - pathology; Oxidative Stress; Random Allocation; Rats; Rats, Wistar; reticulum stress; RNA - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Endocrinopathy; Reticulum; Oxidative stress; Senescence; Rat; Ageing; citokynes; Rodentia; Metabolic diseases; Inflammation; infiltration; reticulum stress; Target tissue resistance; Vertebrata; Mammalia; Development; Insulin resistance; Age
• ISSN: 1381-3455; 1744-4160
• DOI: 10.3109/13813455.2011.577435

Context: Insulin resistance and type 2 Diabetes have been associated to a low grade of inflammation and their prevalence increase with ageing.
Objective: To analyse the development of inflammation in adipose tissue, liver, muscle and hypothalamus during ageing and the effects of caloric restriction.
Materials and methods: We have analysed the expression of inflammatory cytokines (TNFα, IL1-β, IL-12B and IL-6), proteins involved in macrophage recruitment (MCP-1, CCR2), TLR4 and macrophage markers (CD11c, CD11b and arginase1). Immunohistochemistry of macrophages has also been performed.
Results: All studied tissues present signs of inflammation during ageing, but with different pattern and intensity. Caloric restriction decreases the expression of most of inflammatory markers.
Discussion and conclusions: These data indicate a role of adiposity in the development of inflammation and insulin resistance during ageing. Dietetic intervention could be a useful tool to ameliorate the development of inflammation and insulin resistance associated with ageing.