Genome analysis of imperfect fungi: electrophoretic karyotyping and characterization of the nuclear gene coding for glyceraldehyde-3- phosphate dehydrogenase (gpd) of Curvularia lunata

• Published in: Current genetics Vol. 20; no. 1-2; pp. 151 - 155
• Main Authors: Osiewacz, H.D. (Deutsches Krebsforschungszentrum, Heidelberg (Germany). Projektgruppe: Molekularbiologie der Alterungsprozesse), Ridder, R
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-07-1991; Berlin
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; CARIOTIPOS; CARYOTYPE; CURVULARIA; Curvularia lunata; DNA, Fungal; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; Electrophoresis, Gel, Pulsed-Field; Electrophoretic karyotype; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genes, Fungal; Genes. Genome; Genomic Library; GLICERALDEHIDO 3 FOSF DESHIDROG; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROG; GLYCERALDEHYDE 3 PHOSPHATE DESHYDRO; Glyceraldehyde-3-phosphate dehydrogenase (GPD); Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; KARYOTYPES; Karyotyping; Mitosporic Fungi - enzymology; Mitosporic Fungi - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Pulsed-field gel electrophoresis; Sequence Alignment; Nucleotide sequence; Enzyme; Curvularia lunata; Homology; Gene organization; Fungi; Pulsed field electrophoresis; Glyceraldehyde-phosphate dehydrogenase; DNA; Fungi Imperfecti; Molecular cloning; Karyotype; Thallophyta
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00312778

The gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) has been isolated from a genomic library of the filamentous fungus Curvularia lunata. The coding region of this gene consists of 1014 nucleotides and is interrupted by four introns. The gpd gene product shows a high degree of sequence identity with the corresponding proteins of various species belonging to both taxonomically related (e.g., Aspergillus nidulans), as well as more divergent, taxa. Using contour-clamped homogeneous electric field (CHEF) gel electrophoresis eight distinct chromosomal bands have been resolved, with two bands migrating as doublets and one as a triplet. Thus, the total number of chromosomes of C. lunata appears to be 12. The size of the chromosomes ranges from about 1.4 Mb to 4.0 Mb allowing an estimation of the genome to be approximately 29.7 Mb. By hybridization of fractionated chromosomes the gpd gene and the rDNA locus have been localized on individual chromosomes.