Acibenzolar-S-methyl-induced alteration of defence gene expression and enzyme activity in cotton infected with Fusarium oxysporum f. sp. vasinfectum

• Published in: Physiological and molecular plant pathology Vol. 73; no. 6; pp. 175 - 182
• Main Authors: Whan, Jennifer A., Dann, Elizabeth K., Smith, Linda J., Aitken, Elizabeth A.B.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 01-12-2008; Elsevier
• Subjects: 3-Glucanase; Acibenzolar-S-methyl (ASM); Biological and medical sciences; Bion; Chitinase; Cotton; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fusarium oxysporum; Fusarium oxysporum f. sp. vasinfectum; Induced defence; Peroxidase; Phenylalanine ammonia lyase; Phytopathology. Animal pests. Plant and forest protection; Polyphenol oxidase; Real-time RT-PCR; Transcript accumulation; β-1; Real-time RT-PCR; Chitinase; Induced defence; Phenylalanine ammonia lyase; Cotton; Transcript accumulation; Fusarium oxysporum f. sp. vasinfectum; Bion; Peroxidase; β-1; 3-Glucanase; Acibenzolar-S-methyl (ASM); Polyphenol oxidase; Malvaceae; Agricultural chemical product; Phenylalanine ammonia-lyase; Catechol oxidase; Lyases; Systemic; Fungi; Enzymatic activity; Dicotyledones; Angiospermae; Reverse transcription polymerase chain reaction; Organic compounds; Glucanase; Sulfur nitrogen heterocycle; Fusarium oxysporum; Induction; Gene expression; Real time; Fungicide; Carbon-nitrogen lyases; Glycosylases; Infection; Plant pathology; Plant pathogen; Transcription; Gene product; Gossypium; Alteration; Fiber crop; Accumulation; Activator; Fungi Imperfecti; Enzyme; Pesticides; Ammonia-lyases; Peroxidases; Glycosidases; Hydrolases; Defense mechanism; Spermatophyta; Oxidoreductases
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/j.pmpp.2009.06.003

The effect of acibenzolar-S-methyl (ASM) applied in the form of Bion
® as a seed soak or a foliar spray, on transcript accumulation of six defence related genes, and activities of four defence related enzymes, was assessed after cotton was inoculated with
Fusarium oxysporum f. sp.
vasinfectum (
Fov). Significant upregulation of
chitinase class I,
peroxidase, and
β-1, 3-glucanase transcripts occurred in the Bion
® seed soak treatment with
Fov inoculation compared to all other treatments. Bion
® alone did not significantly increase transcripts compared with water control treatments in the majority of cases.
Phenylalanine ammonia lyase transcript in Bion
® treated plants following fungal inoculation was greater than in the respective Bion
® alone treatments, and
osmotin-like protein transcript was significantly greater in inoculated plants sprayed with Bion
® than in all other treatments. Transcripts of
4-coumarate-CoA ligase-like protein were generally lower than for all other genes, and significant upregulation compared with water control occurred in extracts from
Fov inoculated plants receiving no Bion
® treatment, and
Fov inoculated plants cultivated from Bion
® treated seed. The greatest increases in chitinase, β-1, 3-glucanase and peroxidase activity occurred in root and shoot tissue from Bion
® seed treated material inoculated with
Fov. Bion
® treatment alone did not consistently enhance enzyme activities above those measured in water treated controls. Polyphenol oxidase activities were enhanced by various Bion
® or
Fov treatments at 3 days after inoculation only.