Light and acetate regulate a mitochondrial malate dehydrogenase

• Published in: Plant physiology (Bethesda) Vol. 85; no. 1; pp. 124 - 130
• Main Authors: Struck, F, Grolz-Krug, S, Boschek, B, Zetsche, K
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-09-1987
• Subjects: ACETATE; ACETATES; ACETATOS; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AISLAMIENTO (TECNICA); ALGAE; ALGUE; Antibodies; Biological and medical sciences; Cell biochemistry; Cell growth; Cell physiology; CHLOROGONIUM ELONGATUM; Cultured cells; Dehydrogenases; Enzymes; ENZYMIC ACTIVITY; Fundamental and applied biological sciences. Psychology; ISOLATION; ISOLEMENT; LIGHT; LUMIERE; LUZ; MITOCHONDRIA; MITOCHONDRIE; MITOCONDRIA; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Phosphates; Plant cells; Plant physiology and development; Tricarboxylic acid cycle; Light effect; Mitochondria; Enzyme; Algae; Chlorophycophyta; Malate dehydrogenase; Acetate; Localization; Thallophyta
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.85.1.124

A malate dehydrogenase was purified from the unicellular green alga Chlorogonium elongatum Dangeard. The enzyme was localized in the mitochondria by immunogold electron microscopy and was found to be present on the cristae. The concentration of the enzyme is regulated by acetate and light. In cells cultured heterotrophically with acetate as carbon source the activity and the concentration of the enzyme is 5- to 6-fold higher than in autotrophic cells. In mixotrophically cultured cells (light and acetate) the enzyme level attains only half of the value of that in heterotrophic cells. Acetate induces an increase of the enzyme concentration while light has an inhibitory effect on this process.