Gene disruption in Lactobacillus plantarum strain 80 by site-specific recombination: isolation of a mutant strain deficient in conjugated bile salt hydrolase activity

A chloramphenicol-resistance gene (cml) was introduced into the Lactobacillus plantarum gene encoding conjugated bile acid hydrolase (cbh) on a ColE1 replicon. This plasmid which is nonreplicative in Lactobacillus was used to transform L. plantarum strain 80. A homologous double cross-over recombina...

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Bibliographic Details

Published in:	Molecular & general genetics Vol. 239; no. 1-2; p. 269
Main Authors:	Leer, R J, Christiaens, H, Verstraete, W, Peters, L, Posno, M, Pouwels, P H
Format:	Journal Article
Language:	English
Published:	Germany 01-05-1993
Subjects:	Amidohydrolases - biosynthesis Amidohydrolases - genetics Chloramphenicol Resistance - genetics Genes, Bacterial Lactobacillus - enzymology Lactobacillus - genetics Lactobacillus - isolation & purification Mutagenesis, Site-Directed Mutation Recombination, Genetic Restriction Mapping
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