Resveratrol induces the production of reactive oxygen species, interferes with the cell cycle, and inhibits the cell migration of bladder tumour cells with different TP53 status

• Published in: Natural product research Vol. 37; no. 22; pp. 3838 - 3843
• Main Authors: Almeida, Tamires Cunha, Melo, André Sacramento, Lima, Ana Paula Braga, Branquinho, Renata Tupinambá, da Silva, Glenda Nicioli
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Ltd 17-11-2023
• Subjects: Anticancer properties; Antitumor activity; Bladder; Cancer; Cell cycle; Cell migration; Cell proliferation; Cell viability; Cytotoxicity; E-cadherin; Gene expression; Migration inhibition; Migratory species; Molecular modelling; Mutagenicity; Myc protein; Oxygen; p53 Protein; Reactive oxygen species; Resveratrol; Smad4 protein; Tumors
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2151007

Resveratrol is a polyphenolic compound whose antitumor activity has been demonstrated in several types of cancer. However, there are few studies on its molecular mechanisms of action in bladder cancer. Therefore, we aimed to evaluate resveratrol activity in bladder tumour cells with different TP53 gene status. Cytotoxicity, cell proliferation, reactive oxygen species (ROS) production, cell migration, mutagenicity, and CDH1, CTNNBIP1, HAT1, HDAC1, MYC, and SMAD4 gene expression were evaluated. An increase in ROS after resveratrol treatment was accompanied by reduced cell viability and proliferation in all cell lines. In TP53 wild-type cells, the inhibition of cell migration was accompanied by CDH1 and SMAD4 modulation. In TP53 mutated cells, cell migration inhibition with CDH1 and CTNNB1P1 upregulation was observed. In conclusion, resveratrol has antiproliferative effect in bladder tumour cells and its mechanism of action occurred through ROS production, interference with cell cycle, and inhibition of cell migration, independent of TP53 status.