A simple solid injection device for the analyses of Bulbophyllum (Orchidaceae) volatiles

• Published in: Phytochemistry (Oxford) Vol. 50; no. 1; pp. 31 - 34
• Main Authors: da Silva, Ubiratan F, L. Borba, Eduardo, Semir, João, Marsaioli, Anita J
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 01-01-1999; Elsevier
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; B. involutum; B. ipanemense; biochemical techniques; Biological and medical sciences; Bulbophyllum; bulbophyllum involutum; bulbophyllum ipanemense; Bulbophyllum weddellii; chemical constituents of plants; Chemical constitution; flowers; Fundamental and applied biological sciences. Psychology; GC/MS; Orchidaceae; Plant physiology and development; Plants and fungi; pollinators; quantitative analysis; solid injector; species differences; volatile compounds; volatiles; Brazil; Bulbophyllum weddellii; Orchidaceae; B. involutum; B. ipanemense; GC/MS; solid injector; volatiles; Volatile compound; Monocotyledones; Insect pollination; Animal plant relation; Angiospermae; Spermatophyta; Chemical composition; Extraction; Method
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(98)00459-2

The volatile components of three orchid species (
Bulbophyllum weddellii,
B. ipanemense and
B. involutum) were detected using GC/MS. Due to the minute dimension and amount of flowers, special traps and extraction methods were applied and the analyses were finally successful using a special solid sample injector which was constructed in our own laboratory. The chemical composition comparison revealed the high similarity between the volatiles present in
B. weddellii and
B. involutum, which is coherent with the pollinator sharing. On the other hand, the volatiles of
B. ipanemense, a species pollinated by other pollinator species, are different from the two previous species.