A Cell Line Secreting Stimulating Factors for CFU-GEMM Culture

• Published in: Blood Vol. 64; no. 1; pp. 152 - 155
• Main Authors: Myers, C.D., Katz, F.E., Joshi, G., Millar, J.L.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01-07-1984; The Americain Society of Hematology
• Subjects: Animal cells; Biological and medical sciences; Biotechnology; Cell Line; Cell physiology; Culture Media; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Hematopoietic Stem Cells - physiology; Humans; Leukocytes; Methods. Procedures. Technologies; Miscellaneous; Molecular and cellular biology; Phytohemagglutinins - pharmacology; Secretion. Exocytosis; Cell culture; Vertebrata; Cell line; Mammalia; Conditioned medium; Pluripotent cell; Secretion; Hematopoietic cell; Colony stimulating factor
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V64.1.152.152

The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures and show that the properties of this conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM culture systems.