Mitochondrial vulnerability to oxidation in human brain organoids modelling Alzheimer's disease

• Published in: Free radical biology & medicine Vol. 208; pp. 394 - 401
• Main Authors: Holubiec, Mariana I., Alloatti, Matias, Bianchelli, Julieta, Greloni, Francisco, Arnaiz, Cayetana, Gonzalez Prinz, Melina, Fernandez Bessone, Ivan, Pozo Devoto, Victorio, Falzone, Tomas L.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-11-2023
• Subjects: Alzheimer's disease; Amyloid precursor protein; Human brain organoids; Mitochondrial homeostasis; Oxidative distress; Swedish mutation; Thioredoxins; Swedish mutation; Human brain organoids; Oxidative distress; Mitochondrial homeostasis; Thioredoxins; Alzheimer's disease; Amyloid precursor protein
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2023.08.028

Reactive Oxygen Species (ROS) and mitochondrial dysfunction are implicated in the pathogenesis of Alzheimer's disease (AD), a common neurodegenerative disorder characterized by abnormal metabolism of the amyloid precursor protein (APP) in brain tissue. However, the exact mechanism by which abnormal APP leads to oxidative distress remains unclear. Damage to mitochondrial membrane and inhibition of mitochondrial respiration are thought to contribute to the progression of the disease. However, the lack of suitable human models that replicate pathological features, together with impaired cellular pathways, constitutes a major challenge in AD studies. In this work, we induced pluripotency in patient-derived skin fibroblasts carrying the Swedish mutation in App (APPswe), to generate human brain organoids that model AD, and studied redox regulation and mitochondrial homeostasis. We found time-dependent increases in AD-related pathological hallmarks in APPswe brain organoids, including elevated Aβ levels, increased extracellular amyloid deposits, and enhanced tau phosphorylation. Interestingly, using live-imaging spinning-disk confocal microscopy, we found an increase in mitochondrial fragmentation and a significant loss of mitochondrial membrane potential in APPswe brain organoids when subjected to oxidative conditions. Moreover, ratiometric dyes in a live imaging setting revealed a selective increase in mitochondrial superoxide anion and hydrogen peroxide levels in APPswe brain organoids that were coupled to impairments in cytosolic and mitochondrial redoxin protein expression. Our results suggest a selective increase in mitochondrial vulnerability to oxidative conditions in APPswe organoids, indicating that the abnormal metabolism of APP leads to specific changes in mitochondrial homeostasis that enhance the vulnerability to oxidation in AD.
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•We develop a human brain model that exhibit pathological hallmarks of Alzheimer's disease.•APPswe mutation induces mitochondrial-derived oxidative distress in human brain organoids.•Mitochondrial vulnerability to oxidation is exacerbated in a human model of Alzheimer's disease.