Comparison of electrophoretic mobility and membrane sialic acid content of erythrocytes from adult and umbilical cord blood

Determinations of cell electrophoretic mobility at low ionic strength and of ghost sialic acid content show that erythrocytes from umbilical cord blood and from adult donors are identical in these two glycoprotein-related properties. Using a streak deflection electrophoresis in 16.5 mM Tris-acetic a...

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Bibliographic Details
Published in:	Pediatric research Vol. 9; no. 7; pp. 583 - 585
Main Authors:	Luner, S J, Szklarek, D
Format:	Journal Article
Language:	English
Published:	United States 01-07-1975
Subjects:	Adult Cell Membrane - analysis Electrophoresis Erythrocytes - analysis Female Fetal Blood Humans Infant, Newborn Pregnancy Sialic Acids - blood
Online Access:	Get full text
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Summary:	Determinations of cell electrophoretic mobility at low ionic strength and of ghost sialic acid content show that erythrocytes from umbilical cord blood and from adult donors are identical in these two glycoprotein-related properties. Using a streak deflection electrophoresis in 16.5 mM Tris-acetic acid buffer, pH 7.4, no increased streak width indicating electrophoretic heterogeneity could be detected when mixed suspensions of adult and umbilical cord blood erythrocytes were compared with suspensions of adult cells alone. Sialic acid content of 100 nmol/mg protein were obtained for both populations of cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0031-3998 1530-0447
DOI:	10.1203/00006450-197507000-00006