Isolation of Bacillus subtilis transformation-deficient mutants and mapping of competence genes

Isolation of Bacillus subtilis transformation-deficient mutants and mapping of competence genes

We have isolated and characterized 48 Bacillus subtilis competence-deficient mutants. The mutants, obtained by nitrosoguanidine mutagenesis or by insertional mutagenesis with transposon Tn917, had a reduced transformation frequency and a wild-type transduction frequency. The com mutations were mappe...

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Bibliographic Details
Published in:Genetical research Vol. 54; no. 1; p. 1
Main Authors: Mastromei, G, Barberio, C, Pistolesi, S, Polsinelli, M
Format: Journal Article
Language:English
Published: England 01-08-1989
Subjects:
Bacillus subtilis - genetics
Chromosome Mapping
Chromosomes, Bacterial
Genes, Bacterial
Genetic Linkage
Mutation
Transduction, Genetic
Transformation, Bacterial
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Summary:We have isolated and characterized 48 Bacillus subtilis competence-deficient mutants. The mutants, obtained by nitrosoguanidine mutagenesis or by insertional mutagenesis with transposon Tn917, had a reduced transformation frequency and a wild-type transduction frequency. The com mutations were mapped by PBS1 transduction and at least four new com genes have been identified. The mutants were also characterized for their capacity to bind and take up the transforming DNA.
DOI:10.1017/S0016672300028305